Rapid changes in hepatocyte phosphoinositides induced by vasopressin

Vasopressin stimulated a 40% decrease in [³²P]phosphatidylinositol 4,5-bisphosphate and a 15% decrease in [³²P]phosphatidylinositol within 30 s of addition to hepatocytes prelabeled for 60 min with ³²P. In hepatocytes prelabeled with [³H]inositol for 60 min, vasopressin produced 20% breakdown of phosphatidylinositol and 33% breakdown of phosphatidylinositol 4,5-bisphosphate within 30 s. There was a 40% increase in total phosphatidylinositol 4,5-bisphosphate within 30 s of vasopressin addition. Breakdown of phosphatidylinositol accounted for disappearance of 95% of the inositol lipid label. In hepatocytes from rats labeled in vivo with [³H]inositol, vasopressin stimulated 10% loss of labeled phosphatidylinositol. Loss of [³²P]phosphatidylinositol due to vasopressin was followed by reincorporation of label to levels greater than control while ³²P reuptake into phosphatidylinositol 4,5-bisphosphate did not exceed control values. With in vitro [³H]inositol-labeled hepatocytes, loss of label from the phosphoinositides was followed by reuptake of tritium label to control levels. In hepatocytes labeled in vivo with [³H]inositol, reuptake of [³H]inositol label did not occur. These data indicate that the hormone-sensitive pool of hepatocyte phosphoinositides can be labeled by both in vitro and in vivo procedures. Vasopressin induces a rapid decrease of labeled phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate within 30 s.