Rat amnion type IV collagen composition and metabolism: Implications for membrane breakdown

Hanqin Lei, Raghu Kalluri, Emma E. Furth, Andrew H. Baker, Jerome F. Strauss

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We report here that rat amnion type IV collagens are composed primarily of α1(IV) and α2(IV) chains. Amnion basement membrane collagens were more sensitive to degradation by collagenases than were adult rat kidney basement membrane collagens, which are enriched in α3(IV), α4(IV), and α6(IV) chains. Amnion type IV collagen content per unit of protein was markedly reduced by Day 21 of pregnancy, the day of delivery. Increased amnion levels of matrix metalloproteinase (MMP)-2 and MMP-9, gelatinases that degrade type IV collagen, were found by Day 21, suggesting that collagen breakdown was responsible, in part, for the decline in amnion type IV collagen. Infection of organ cultures of Day 18 rat amnions with a recombinant adenovirus expressing MMP-9 (AdMMP-9) caused release of collagen fragments detected as hydroxyproline in the culture fluid, amnion cell detachment, and apoptosis. The AdMMP-9-induced apoptosis was prevented by the MMP inhibitor batimastat. These findings suggest that MMPs are implicated in anoikis and apoptotic death of amnion cells, and may be part of a complex program of fetal membrane remodeling that occurs before delivery.

Original languageEnglish (US)
Pages (from-to)176-182
Number of pages7
JournalBiology of Reproduction
Volume60
Issue number1
DOIs
StatePublished - Jan 14 1999

Fingerprint

Amnion
Collagen Type IV
Membranes
Collagen
Matrix Metalloproteinase 9
Matrix Metalloproteinases
Basement Membrane
Adenoviridae
Anoikis
Apoptosis
Extraembryonic Membranes
Gelatinases
Matrix Metalloproteinase Inhibitors
Organ Culture Techniques
Matrix Metalloproteinase 2
Hydroxyproline
Collagenases
Cell Death
Kidney
Pregnancy

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rat amnion type IV collagen composition and metabolism : Implications for membrane breakdown. / Lei, Hanqin; Kalluri, Raghu; Furth, Emma E.; Baker, Andrew H.; Strauss, Jerome F.

In: Biology of Reproduction, Vol. 60, No. 1, 14.01.1999, p. 176-182.

Research output: Contribution to journalArticle

Lei, Hanqin ; Kalluri, Raghu ; Furth, Emma E. ; Baker, Andrew H. ; Strauss, Jerome F. / Rat amnion type IV collagen composition and metabolism : Implications for membrane breakdown. In: Biology of Reproduction. 1999 ; Vol. 60, No. 1. pp. 176-182.
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