Reduced Human Germ Cell-Less (HGCL) Expression in Azoospermic Men with Severe Germinal Cell Impairment

Sandra E. Kleiman, Leah Yogev, Einav Nili Gal-Yam, Ron Hauser, Ronni Gamzu, Amnon Botchan, Gedalia Paz, Haim Yavetz, Batia Bar Shira Maymon, Letizia Schreiber, Shlomit Barzilai, Ninette Amariglio, Gideon Rechavi, Amos J. Simon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Germ cell-less (GCL) protein is a nuclear envelope protein highly conserved between the mammalian and Drosophila orthologues. In Drosophila, maternal GCL protein is required to establish the germ lineage during embryonic development. In mammals, it is suggested that the GCL function is mainly in spermatogenesis and that it might be related to the ability of mouse GCL to repress transcription. Using reverse transcriptase-polymerase chain reaction analyses, we investigated the role of human GCL (HGCL) in spermatogenesis by studying its expression in the testicular tissue of 67 azoospermic men with normal karyotype and no Y-chromosome microdetetion. Their testicular biopsy specimens underwent meticulous histological and cytological analysis as well as molecular analysis with various markers of spermatogenesis (RBM1, DAZ, and CDY1). The rate of X-Y and 18 chromosome bivalent formation during meiosis was additionally assessed in 22 of these biopsy specimens and correlated to HGCL expression. Expression of HGCL was affected in parallel with the severity of testicular impairment found. Defective sperm motility was associated with the absence of HGCL, Nevertheless, the absence of HGCL expression did not influence the normal process of chromosome bivalent formation in meiosis. Our results suggest that HGCL is not essential for the chromosomal events of meiosis but might be involved in later aspects of spermatogenesis.

Original languageEnglish (US)
Pages (from-to)670-675
Number of pages6
JournalJournal of andrology
Volume24
Issue number5
DOIs
StatePublished - Jan 1 2003

Fingerprint

Germ Cells
Spermatogenesis
Meiosis
Y Chromosome
Drosophila
Biopsy
Sperm Motility
Nuclear Envelope
X Chromosome
Nuclear Proteins
Reverse Transcriptase Polymerase Chain Reaction
Karyotype
Embryonic Development
Mammals
Proteins
Chromosomes
Mothers

Keywords

  • HGCL and chromosome bivalent formation
  • Markers of spermatogenesis
  • Motility impairments
  • Spermatogenesis impairments
  • Testicular HGCL expression

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Reproductive Medicine
  • Endocrinology
  • Urology

Cite this

Reduced Human Germ Cell-Less (HGCL) Expression in Azoospermic Men with Severe Germinal Cell Impairment. / Kleiman, Sandra E.; Yogev, Leah; Gal-Yam, Einav Nili; Hauser, Ron; Gamzu, Ronni; Botchan, Amnon; Paz, Gedalia; Yavetz, Haim; Maymon, Batia Bar Shira; Schreiber, Letizia; Barzilai, Shlomit; Amariglio, Ninette; Rechavi, Gideon; Simon, Amos J.

In: Journal of andrology, Vol. 24, No. 5, 01.01.2003, p. 670-675.

Research output: Contribution to journalArticle

Kleiman, SE, Yogev, L, Gal-Yam, EN, Hauser, R, Gamzu, R, Botchan, A, Paz, G, Yavetz, H, Maymon, BBS, Schreiber, L, Barzilai, S, Amariglio, N, Rechavi, G & Simon, AJ 2003, 'Reduced Human Germ Cell-Less (HGCL) Expression in Azoospermic Men with Severe Germinal Cell Impairment', Journal of andrology, vol. 24, no. 5, pp. 670-675. https://doi.org/10.1002/j.1939-4640.2003.tb02725.x
Kleiman, Sandra E. ; Yogev, Leah ; Gal-Yam, Einav Nili ; Hauser, Ron ; Gamzu, Ronni ; Botchan, Amnon ; Paz, Gedalia ; Yavetz, Haim ; Maymon, Batia Bar Shira ; Schreiber, Letizia ; Barzilai, Shlomit ; Amariglio, Ninette ; Rechavi, Gideon ; Simon, Amos J. / Reduced Human Germ Cell-Less (HGCL) Expression in Azoospermic Men with Severe Germinal Cell Impairment. In: Journal of andrology. 2003 ; Vol. 24, No. 5. pp. 670-675.
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AU - Gal-Yam, Einav Nili

AU - Hauser, Ron

AU - Gamzu, Ronni

AU - Botchan, Amnon

AU - Paz, Gedalia

AU - Yavetz, Haim

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AU - Simon, Amos J.

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