Regulation of lymphocyte tumor necrosis factor receptors by IL-2

L. B. Owen-Schaub, W. L. Crump, G. I. Morin, Elizabeth A Grimm

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Activated lymphocytes are known to express TNF receptors. The precise stimuli involved in induction and regulation of these receptors have not been elucidated. Our findings demonstrate that IL-2, alone and in serum-free conditions, can trigger and regulate TNF receptor expression on normal lymphocytes. Flow cytometric analyses demonstrated that the receptor was rapidly induced on CD4, CD8, and CD16+ cells after IL-2 stimulation. Receptors increased with culture duration, became maximal between days 5 and 9, and were maintained for 18 to 20 days in the presence of IL-2. By using 125I-TNF and FITC-TNF binding, we present evidence that IL-2 concentration determines the magnitude of lymphoid TNF receptor expression - influencing both the percentage TNF-positive cells within the population and the number of receptors/cell. Collectively, our results are persuasive for consideration of IL-2 as a central mediator in the regulation of lymphocyte TNF receptors.

Original languageEnglish (US)
Pages (from-to)2236-2241
Number of pages6
JournalJournal of Immunology
Volume143
Issue number7
StatePublished - 1989

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Receptors, Tumor Necrosis Factor, Type II
Tumor Necrosis Factor Receptors
Interleukin-2
Lymphocytes
Fluorescein-5-isothiocyanate
Cell Count
Serum
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Owen-Schaub, L. B., Crump, W. L., Morin, G. I., & Grimm, E. A. (1989). Regulation of lymphocyte tumor necrosis factor receptors by IL-2. Journal of Immunology, 143(7), 2236-2241.

Regulation of lymphocyte tumor necrosis factor receptors by IL-2. / Owen-Schaub, L. B.; Crump, W. L.; Morin, G. I.; Grimm, Elizabeth A.

In: Journal of Immunology, Vol. 143, No. 7, 1989, p. 2236-2241.

Research output: Contribution to journalArticle

Owen-Schaub, LB, Crump, WL, Morin, GI & Grimm, EA 1989, 'Regulation of lymphocyte tumor necrosis factor receptors by IL-2', Journal of Immunology, vol. 143, no. 7, pp. 2236-2241.
Owen-Schaub, L. B. ; Crump, W. L. ; Morin, G. I. ; Grimm, Elizabeth A. / Regulation of lymphocyte tumor necrosis factor receptors by IL-2. In: Journal of Immunology. 1989 ; Vol. 143, No. 7. pp. 2236-2241.
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