Reprogramming mouse fibroblasts with piggyBac transposons

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

In 2006, Shinya Yamanaka and his student Kazutoshi Takahashi showed that the expression of only four specific genes is sufficient to reprogram fully differentiated somatic cells into pluripotent stem cells. These cells, termed induced pluripotent stem (iPS) cells, share many of their characteristics with embryonic stem (ES) cells. In this protocol, we describe one of the simplest ways of generating iPS cells from mouse fibroblasts. It combines an efficient transposon-mediated transfection and the tetracy-cline-inducible system to control the expression of the Yamanaka reprogramming factors.

Original languageEnglish (US)
Pages (from-to)827-830
Number of pages4
JournalCold Spring Harbor protocols
Volume2017
Issue number10
DOIs
StatePublished - Oct 2017

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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