TY - JOUR
T1 - Reprogramming mouse fibroblasts with piggyBac transposons
AU - Behringer, Richard
AU - Gertsenstein, Marina
AU - Vintersten Nagy, Kristina
AU - Nagy, Andras
N1 - Publisher Copyright:
© 2017 Cold Spring Harbor Laboratory Press.
PY - 2017/10
Y1 - 2017/10
N2 - In 2006, Shinya Yamanaka and his student Kazutoshi Takahashi showed that the expression of only four specific genes is sufficient to reprogram fully differentiated somatic cells into pluripotent stem cells. These cells, termed induced pluripotent stem (iPS) cells, share many of their characteristics with embryonic stem (ES) cells. In this protocol, we describe one of the simplest ways of generating iPS cells from mouse fibroblasts. It combines an efficient transposon-mediated transfection and the tetracy-cline-inducible system to control the expression of the Yamanaka reprogramming factors.
AB - In 2006, Shinya Yamanaka and his student Kazutoshi Takahashi showed that the expression of only four specific genes is sufficient to reprogram fully differentiated somatic cells into pluripotent stem cells. These cells, termed induced pluripotent stem (iPS) cells, share many of their characteristics with embryonic stem (ES) cells. In this protocol, we describe one of the simplest ways of generating iPS cells from mouse fibroblasts. It combines an efficient transposon-mediated transfection and the tetracy-cline-inducible system to control the expression of the Yamanaka reprogramming factors.
UR - http://www.scopus.com/inward/record.url?scp=85030649981&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85030649981&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot092627
DO - 10.1101/pdb.prot092627
M3 - Article
C2 - 28974653
AN - SCOPUS:85030649981
SN - 1940-3402
VL - 2017
SP - 827
EP - 830
JO - Cold Spring Harbor protocols
JF - Cold Spring Harbor protocols
IS - 10
ER -