Retinoic acid differentiation of HL-60 cells promotes cytoskeletal polarization

Ada L. Olins, Harald Herrmann, Peter Lichter, Donald E. Olins

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Retinoic acid (RA) treatment of HL-60 cells in vitro induces granulocytic differentiation, involving reorganization of the nucleus and cytoplasm, development of chemoattractant-directed migration, and eventual apoptosis. The present studies with HL-60/S4 cells document that major elements of the cytoskeleton are changed: actin increases by 50%; vimentin decreases by more than 95%. The cellular content of α-tubulin does not significantly change; but the centrosomal-microtubule (MT) array moves away from the lobulating nucleus. Cytoskeletal-modifying chemicals modulate this polarized reorganization: Taxol and cytochalasin D enhance centrosome movement; nocodazole reverses it. Cytoskeletal-modifying chemicals do not appear to affect nuclear lobulation or the integrity of envelope-limited chromatin sheets (ELCS). Employing bcl-2-overexpressing HL-60 cells permitted demonstration of nuclear lobulation, ELCS formation, and centrosome-MT movement concomitantly during RA-induced differentiation, implying independence between the cellular reorganization and apoptotic programs. RA appears to promote an inherent potential in HL-60 cells for cytoskeletal polarization, likely to be important for chemoattractant-directed cell migration, an established characteristic of mature granulocytes. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)130-142
Number of pages13
JournalExperimental Cell Research
Volume254
Issue number1
DOIs
StatePublished - Jan 10 2000

Fingerprint

HL-60 Cells
Tretinoin
Centrosome
Chemotactic Factors
Microtubules
Chromatin
Nocodazole
Cytochalasin D
Nuclear Envelope
Vimentin
Tubulin
Paclitaxel
Actin Cytoskeleton
Granulocytes
Cell Movement
Cytoplasm
Apoptosis

Keywords

  • Centrosome microtubules
  • Granulocytes
  • Nuclear envelope
  • Retinoic acid

ASJC Scopus subject areas

  • Cell Biology

Cite this

Retinoic acid differentiation of HL-60 cells promotes cytoskeletal polarization. / Olins, Ada L.; Herrmann, Harald; Lichter, Peter; Olins, Donald E.

In: Experimental Cell Research, Vol. 254, No. 1, 10.01.2000, p. 130-142.

Research output: Contribution to journalArticle

Olins, Ada L. ; Herrmann, Harald ; Lichter, Peter ; Olins, Donald E. / Retinoic acid differentiation of HL-60 cells promotes cytoskeletal polarization. In: Experimental Cell Research. 2000 ; Vol. 254, No. 1. pp. 130-142.
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