Retrograde transport of endocytosed Shiga toxin to the endoplasmic reticulum

Kirsten Sandvig, Øystein Garred, Kristian Prydz, Juri V. Kozlov, Steen H. Hansen, Bo Van Deurs

Research output: Contribution to journalArticle

343 Citations (Scopus)

Abstract

SHIGA toxin and some other protein toxins that act on targets in the cytosol have previously been shown to enter the trans-Golgi network 1-9 . Transport by this route may be necessary for translocation of the toxin to the cytosol and for intoxication 5-9 , but it is not known whether the enzymatically active part of the toxins actually enters the cytosol from the fraiw-Golgi network. It has been suggested that such toxins are transported in a retrograde manner to the endoplasmic reticulum and that translocation occurs in this organelle 10 , but retrograde transport of endocytosed material beyond the trans-Golgi network has never been demonstrated. Here we show that in butyric acid-treated A431 cells endocytosed Shiga toxin is not only transported to the trans-Golgi network, but also to all Golgi stacks, to the endoplasmic reticulum and to the nuclear envelope. Furthermore, butyric acid sensitizes the cells to Shiga toxin, which is consistent with the possibility that retrograde transport is required for translocation of the toxin to the cytosol.

Original languageEnglish (US)
Pages (from-to)510-512
Number of pages3
JournalNature
Volume358
Issue number6386
DOIs
StatePublished - Jan 1 1992

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Shiga Toxin
Endocytosis
trans-Golgi Network
Endoplasmic Reticulum
Cytosol
Butyric Acid
Nuclear Envelope
Organelles
Proteins

ASJC Scopus subject areas

  • General

Cite this

Sandvig, K., Garred, Ø., Prydz, K., Kozlov, J. V., Hansen, S. H., & Van Deurs, B. (1992). Retrograde transport of endocytosed Shiga toxin to the endoplasmic reticulum. Nature, 358(6386), 510-512. https://doi.org/10.1038/358510a0

Retrograde transport of endocytosed Shiga toxin to the endoplasmic reticulum. / Sandvig, Kirsten; Garred, Øystein; Prydz, Kristian; Kozlov, Juri V.; Hansen, Steen H.; Van Deurs, Bo.

In: Nature, Vol. 358, No. 6386, 01.01.1992, p. 510-512.

Research output: Contribution to journalArticle

Sandvig, K, Garred, Ø, Prydz, K, Kozlov, JV, Hansen, SH & Van Deurs, B 1992, 'Retrograde transport of endocytosed Shiga toxin to the endoplasmic reticulum', Nature, vol. 358, no. 6386, pp. 510-512. https://doi.org/10.1038/358510a0
Sandvig K, Garred Ø, Prydz K, Kozlov JV, Hansen SH, Van Deurs B. Retrograde transport of endocytosed Shiga toxin to the endoplasmic reticulum. Nature. 1992 Jan 1;358(6386):510-512. https://doi.org/10.1038/358510a0
Sandvig, Kirsten ; Garred, Øystein ; Prydz, Kristian ; Kozlov, Juri V. ; Hansen, Steen H. ; Van Deurs, Bo. / Retrograde transport of endocytosed Shiga toxin to the endoplasmic reticulum. In: Nature. 1992 ; Vol. 358, No. 6386. pp. 510-512.
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