Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer

J. A. Roth; D. Nguyen; D. D. Lawrence; B. L. Kemp; C. H. Carrasco; D. Z. Ferson; W. K. Hong; R. Komaki; J. J. Lee; J. C. Nesbitt; K. M.W. Pisters; J. B. Putnam; R. Schea; D. M. Shin; G. L. Walsh; M. M. Dolormente; C. I. Han; F. D. Martin; N. Yen; K. Xu; L. C. Stephens; T. J. Mcdonnell; T. Mukhopadhyay; D. Cai

doi:10.1038/nm0996-985

Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer

J. A. Roth, D. Nguyen, D. D. Lawrence, B. L. Kemp, C. H. Carrasco, D. Z. Ferson, W. K. Hong, R. Komaki, J. J. Lee, J. C. Nesbitt, K. M.W. Pisters, J. B. Putnam, R. Schea, D. M. Shin, G. L. Walsh, M. M. Dolormente, C. I. Han, F. D. Martin, N. Yen, K. XuL. C. Stephens, T. J. Mcdonnell, T. Mukhopadhyay, D. Cai

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Abstract

A retroviral vector containing the wild-type p53 gene under control of a β-actin promoter was produced to mediate transfer of wild-type p53 into human non-small cell lung cancers by direct injection. Nine patients whose conventional treatments failed were entered into the study. No clinically significant vector-related toxic effects were noted up to five months after treatment. In situ hybridization and DNA polymerase chain reaction showed vector-p53 sequences in posttreatment biopsies. Apoptosis (programmed cell death) was more frequent in posttreatment biopsies than in pretreatment biopsies. Tumor regression was noted in three patients, and tumor growth stabilized in three other patients.

Original language	English (US)
Pages (from-to)	985-991
Number of pages	7
Journal	Nature medicine
Volume	2
Issue number	9
DOIs	https://doi.org/10.1038/nm0996-985
State	Published - Sep 1996

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1038/nm0996-985

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Roth, J. A., Nguyen, D., Lawrence, D. D., Kemp, B. L., Carrasco, C. H., Ferson, D. Z., Hong, W. K., Komaki, R., Lee, J. J., Nesbitt, J. C., Pisters, K. M. W., Putnam, J. B., Schea, R., Shin, D. M., Walsh, G. L., Dolormente, M. M., Han, C. I., Martin, F. D., Yen, N., ... Cai, D. (1996). Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer. Nature medicine, 2(9), 985-991. https://doi.org/10.1038/nm0996-985

Roth, JA, Nguyen, D, Lawrence, DD, Kemp, BL, Carrasco, CH, Ferson, DZ, Hong, WK, Komaki, R, Lee, JJ, Nesbitt, JC, Pisters, KMW, Putnam, JB, Schea, R, Shin, DM, Walsh, GL, Dolormente, MM, Han, CI, Martin, FD, Yen, N, Xu, K, Stephens, LC, Mcdonnell, TJ, Mukhopadhyay, T & Cai, D 1996, 'Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer', Nature medicine, vol. 2, no. 9, pp. 985-991. https://doi.org/10.1038/nm0996-985

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title = "Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer",

abstract = "A retroviral vector containing the wild-type p53 gene under control of a β-actin promoter was produced to mediate transfer of wild-type p53 into human non-small cell lung cancers by direct injection. Nine patients whose conventional treatments failed were entered into the study. No clinically significant vector-related toxic effects were noted up to five months after treatment. In situ hybridization and DNA polymerase chain reaction showed vector-p53 sequences in posttreatment biopsies. Apoptosis (programmed cell death) was more frequent in posttreatment biopsies than in pretreatment biopsies. Tumor regression was noted in three patients, and tumor growth stabilized in three other patients.",

author = "Roth, {J. A.} and D. Nguyen and Lawrence, {D. D.} and Kemp, {B. L.} and Carrasco, {C. H.} and Ferson, {D. Z.} and Hong, {W. K.} and R. Komaki and Lee, {J. J.} and Nesbitt, {J. C.} and Pisters, {K. M.W.} and Putnam, {J. B.} and R. Schea and Shin, {D. M.} and Walsh, {G. L.} and Dolormente, {M. M.} and Han, {C. I.} and Martin, {F. D.} and N. Yen and K. Xu and Stephens, {L. C.} and Mcdonnell, {T. J.} and T. Mukhopadhyay and D. Cai",

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AU - Carrasco, C. H.

AU - Ferson, D. Z.

AU - Hong, W. K.

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AU - Pisters, K. M.W.

AU - Putnam, J. B.

AU - Schea, R.

AU - Shin, D. M.

AU - Walsh, G. L.

AU - Dolormente, M. M.

AU - Han, C. I.

AU - Martin, F. D.

AU - Yen, N.

AU - Xu, K.

AU - Stephens, L. C.

AU - Mcdonnell, T. J.

AU - Mukhopadhyay, T.

AU - Cai, D.

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Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer

Abstract

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