Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail

Ken Ichi Nakamura, Akiko Fujita, Takahiko Murata, Go Watanabe, Chisato Mori, Jun Fujita, Naoki Watanabe, Toshimasa Ishizaki, Osamu Yoshida, Shuh Narumiya

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Tissue distribution and cellular localization of rhophilin, a 71 kDa Rho-binding protein, were examined in mice. Rhophilin mRNA was highly expressed in adult testis, but was absent in the testis of W/W(V) mice deficient in germ cells. An anti-rhophilin antibody detected a band of an expected size in sperm extracts, which was enriched in the tail fraction. Immunofluorescence analysis revealed two lines of striated staining running in parallel in the principal piece of the sperm tail. These results suggest that rhophilin is expressed in germ cells and localized in the fibrous sheath of the sperm tail. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)9-13
Number of pages5
JournalFEBS Letters
Volume445
Issue number1
DOIs
StatePublished - Feb 19 1999

Fingerprint

Sperm Tail
Monomeric GTP-Binding Proteins
Testis
Carrier Proteins
Germ Cells
Cells
Tissue Distribution
Running
Fluorescent Antibody Technique
Spermatozoa
Tail
Anti-Idiotypic Antibodies
Tissue
Staining and Labeling
Messenger RNA
rhophilin
Antibodies

Keywords

  • Fibrous sheath
  • Rho
  • Rhophilin
  • Sperm
  • Sperm tail
  • Testis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail. / Nakamura, Ken Ichi; Fujita, Akiko; Murata, Takahiko; Watanabe, Go; Mori, Chisato; Fujita, Jun; Watanabe, Naoki; Ishizaki, Toshimasa; Yoshida, Osamu; Narumiya, Shuh.

In: FEBS Letters, Vol. 445, No. 1, 19.02.1999, p. 9-13.

Research output: Contribution to journalArticle

Nakamura, KI, Fujita, A, Murata, T, Watanabe, G, Mori, C, Fujita, J, Watanabe, N, Ishizaki, T, Yoshida, O & Narumiya, S 1999, 'Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail', FEBS Letters, vol. 445, no. 1, pp. 9-13. https://doi.org/10.1016/S0014-5793(99)00087-3
Nakamura, Ken Ichi ; Fujita, Akiko ; Murata, Takahiko ; Watanabe, Go ; Mori, Chisato ; Fujita, Jun ; Watanabe, Naoki ; Ishizaki, Toshimasa ; Yoshida, Osamu ; Narumiya, Shuh. / Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail. In: FEBS Letters. 1999 ; Vol. 445, No. 1. pp. 9-13.
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AU - Mori, Chisato

AU - Fujita, Jun

AU - Watanabe, Naoki

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