Abstract
Y-box protein 1 (YB-1) is a multifunctional DNA/RNA-binding protein that regulates transcription and translation. The specificity of YB-1's RNA binding and its consequences are unknown. Because expression and subcellular localization of YB-1 have been reported to be important in breast cancer, we determined the specificity and functional impact of YB-1 mRNA-binding in MCF7 breast cancer cells. We used YB-1 antibodies to immunoprecipitate YB-1 and microarray profiling to compare YB-1-bound and total poly(A) RNA. We demonstrated that YB-1 mRNA-binding was preferential. Transcript sequences significantly associated with this binding had high GC content. Selected YB-1 mRNA-binding targets were confirmed by QRT-PCR. However, downregulation of YB-1 levels by siRNA did not affect their RNA or protein expression. Thus, YB-1 has RNA-binding specificity; however, YB-1 binding does not necessarily regulate the stability or translation of its mRNA targets. Further study is needed to determine the functional consequences of selective YB-1 mRNA binding.
Original language | English (US) |
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Pages (from-to) | 59-64 |
Number of pages | 6 |
Journal | RNA Biology |
Volume | 6 |
Issue number | 1 |
DOIs | |
State | Published - 2009 |
Keywords
- Breast cancer
- Microarray
- Poly(A) RNA
- RNA-binding
- Translation elongation
- YB-1
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
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- Advanced Technology Genomics Core
- Bioinformatics Shared Resource