RNA-binding specificity of Y-box protein 1

Jin Jiang Dong, Argun Akcakanat, David N. Stivers, Jiexin Zhang, Doyil Kim, Funda Meric-Bernstam

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Y-box protein 1 (YB-1) is a multifunctional DNA/RNA-binding protein that regulates transcription and translation. The specificity of YB-1's RNA binding and its consequences are unknown. Because expression and subcellular localization of YB-1 have been reported to be important in breast cancer, we determined the specificity and functional impact of YB-1 mRNA-binding in MCF7 breast cancer cells. We used YB-1 antibodies to immunoprecipitate YB-1 and microarray profiling to compare YB-1-bound and total poly(A) RNA. We demonstrated that YB-1 mRNA-binding was preferential. Transcript sequences significantly associated with this binding had high GC content. Selected YB-1 mRNA-binding targets were confirmed by QRT-PCR. However, downregulation of YB-1 levels by siRNA did not affect their RNA or protein expression. Thus, YB-1 has RNA-binding specificity; however, YB-1 binding does not necessarily regulate the stability or translation of its mRNA targets. Further study is needed to determine the functional consequences of selective YB-1 mRNA binding.

Original languageEnglish (US)
Pages (from-to)59-64
Number of pages6
JournalRNA Biology
Volume6
Issue number1
DOIs
StatePublished - 2009

Keywords

  • Breast cancer
  • Microarray
  • Poly(A) RNA
  • RNA-binding
  • Translation elongation
  • YB-1

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

MD Anderson CCSG core facilities

  • Advanced Technology Genomics Core
  • Bioinformatics Shared Resource

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