Role of Mac-1 (CD11b/CD18) in neutrophil functions in vivo

H. Lu, B. J. Hughes, J. Smolen, C. M. Ballantyne, L. Tang, A. L. Beaudet, C. W. Smith

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Abstract

In previous studies we obtained evidence that Mac-1 is not necessary for peritoneal emigration of neutrophils in mice deficient in CD11b (Mac-1 -/-) or following administration of blocking antibody to CD11b (Lu et al. FASEB J., A1281, '96). LFA-1 (CD11a/CD18) on neutrophils appears to be sufficient for peritoneal emigration. In the current study we investigated the role of Mac-1 in neutrophil adhesion to fibrinogen, and degranulation in vivo. Fibrinogen-coated Mylar discs were implanted in the peritoneal cavity of Mac-1-/- or Mac-1+/+ mice, and 16 hours later discs and inflammatory exudate were harvested. While neutrophil influx was not significantly different, neutrophil adhesion to the discs was reduced >90% (p<0.01) in the Mac-1-/- mice. Neutrophil degranulation was investigated by measuring β-glucuronidase (a constituent of primary granules) in both peripheral and exudate neutrophils after 4 hours of thioglycollate induced peritonitis. Peritoneal exudate neutrophils in the Mac-1-/- mice retained more than 75% of their β-glucuronidase compared to peripheral neutrophils, while wild-type (+/+) litter mates retained 30% of the enzyme (p<0.05). These results support the conclusion that emigration of neutrophils into the peritoneal cavity does not require Mac-1-dependent adhesion, but the degranulation that is associated with neutrophil extravasation is heavily dependent on Mac-1 adhesion.

Original languageEnglish (US)
Pages (from-to)A338
JournalFASEB Journal
Volume11
Issue number3
StatePublished - Dec 1 1997

Fingerprint

neutrophils
Neutrophils
Adhesion
Glucuronidase
Fibrinogen
Thioglycolates
adhesion
Lymphocyte Function-Associated Antigen-1
Emigration and Immigration
Blocking Antibodies
Exudates and Transudates
Peritoneal Cavity
mice
fibrinogen
Enzymes
peritonitis
Peritonitis
granules
antibodies
enzymes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Lu, H., Hughes, B. J., Smolen, J., Ballantyne, C. M., Tang, L., Beaudet, A. L., & Smith, C. W. (1997). Role of Mac-1 (CD11b/CD18) in neutrophil functions in vivo. FASEB Journal, 11(3), A338.

Role of Mac-1 (CD11b/CD18) in neutrophil functions in vivo. / Lu, H.; Hughes, B. J.; Smolen, J.; Ballantyne, C. M.; Tang, L.; Beaudet, A. L.; Smith, C. W.

In: FASEB Journal, Vol. 11, No. 3, 01.12.1997, p. A338.

Research output: Contribution to journalArticle

Lu, H, Hughes, BJ, Smolen, J, Ballantyne, CM, Tang, L, Beaudet, AL & Smith, CW 1997, 'Role of Mac-1 (CD11b/CD18) in neutrophil functions in vivo', FASEB Journal, vol. 11, no. 3, pp. A338.
Lu H, Hughes BJ, Smolen J, Ballantyne CM, Tang L, Beaudet AL et al. Role of Mac-1 (CD11b/CD18) in neutrophil functions in vivo. FASEB Journal. 1997 Dec 1;11(3):A338.
Lu, H. ; Hughes, B. J. ; Smolen, J. ; Ballantyne, C. M. ; Tang, L. ; Beaudet, A. L. ; Smith, C. W. / Role of Mac-1 (CD11b/CD18) in neutrophil functions in vivo. In: FASEB Journal. 1997 ; Vol. 11, No. 3. pp. A338.
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