TY - GEN
T1 - SERS and micro-sers analysis of doxorubicin interaction in vitro and in living human cancer cells
AU - Morjani, Hamid
AU - Sharonov, Sergei
AU - Manfait, Michel
AU - Sokolov, Kostantin
AU - Nabiev, Igor
N1 - Publisher Copyright:
© 1992 IEEE.
PY - 1992
Y1 - 1992
N2 - A new technique for selective measurement of small amounts of antitumor drugs in the nucleus and cytoplasm of a living cancer cell, based on surface-enhanced Raman spectroscopy (SERS), is proposed. The ability to detect SERS signals from very diluted (up to 10-10 M) solutions of doxorubicin (DOX) as well as from its complex with DNA in vitro and in living cells, has been demonstrated. SERS spectra were obtained from a population as well as.from single living erythroleukemia K562 cells treated with DOX. The SERS spectrum obtained from nucleus of living cells correlated well with the spectrum of the in vitro complex of DOX with Calf thymus DNA. The spectrum obtained from cytoplasm is different from the spectrum of free DOX. This means that DOX in the cytoplasm has some kind of target different from its target (DNA) in the cell nucleus.
AB - A new technique for selective measurement of small amounts of antitumor drugs in the nucleus and cytoplasm of a living cancer cell, based on surface-enhanced Raman spectroscopy (SERS), is proposed. The ability to detect SERS signals from very diluted (up to 10-10 M) solutions of doxorubicin (DOX) as well as from its complex with DNA in vitro and in living cells, has been demonstrated. SERS spectra were obtained from a population as well as.from single living erythroleukemia K562 cells treated with DOX. The SERS spectrum obtained from nucleus of living cells correlated well with the spectrum of the in vitro complex of DOX with Calf thymus DNA. The spectrum obtained from cytoplasm is different from the spectrum of free DOX. This means that DOX in the cytoplasm has some kind of target different from its target (DNA) in the cell nucleus.
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U2 - 10.1109/IEMBS.1992.5760989
DO - 10.1109/IEMBS.1992.5760989
M3 - Conference contribution
AN - SCOPUS:85067042304
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 330
EP - 331
BT - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 1992
A2 - Coatrieux, Jean Louis
A2 - Plonsey, Robert
A2 - Morucci, Jean Pierre
A2 - Laxminarayan, Swamy
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 14th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 1992
Y2 - 29 October 1992 through 1 November 1992
ER -