Single-cell mass cytometry of acute myeloid leukemia and leukemia stem/progenitor cells

Zhihong Zeng, Marina Konopleva, Michael Andreeff

Research output: Chapter in Book/Report/Conference proceedingChapter

25 Scopus citations

Abstract

Mass cytometry time-of-flight (CyTOF) empowers us to understand acute myeloid leukemia (AML) biology at the single-cell level. This technology, combined with advanced data-analysis methods, enables identification and characterization of the rare AML stem/progenitor cells that play key roles in drug resistance and AML relapse. Here we provide a protocol for AML sample preparation for CyTOF and keynotes emphasizing critical steps in and troubleshooting strategies for this procedure.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages75-86
Number of pages12
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1633
ISSN (Print)1064-3745

Keywords

  • Acute myeloid leukemia
  • Mass cytometry time-of-flight

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

MD Anderson CCSG core facilities

  • Flow Cytometry and Cellular Imaging Facility

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