SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing

Sifan Chen, Maximilian Felix Blank, Aishwarya Iyer, Bingding Huang, Lin Wang, Ingrid Grummt, Renate Voit

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

SIRT7 is an NAD+-dependent protein deacetylase with important roles in ribosome biogenesis and cell proliferation. Previous studies have established that SIRT7 is associated with RNA polymerase I, interacts with pre-ribosomal RNA (rRNA) and promotes rRNA synthesis. Here we show that SIRT7 is also associated with small nucleolar RNP (snoRNPs) that are involved in pre-rRNA processing and rRNA maturation. Knockdown of SIRT7 impairs U3 snoRNA dependent early cleavage steps that are necessary for generation of 18S rRNA. Mechanistically, SIRT7 deacetylates U3-55k, a core component of the U3 snoRNP complex, and reversible acetylation of U3-55k modulates the association of U3-55k with U3 snoRNA. Deacetylation by SIRT7 enhances U3-55k binding to U3 snoRNA, which is a prerequisite for pre-rRNA processing. Under stress conditions, SIRT7 is released from nucleoli, leading to hyperacetylation of U3-55k and attenuation of pre-rRNA processing. The results reveal a multifaceted role of SIRT7 in ribosome biogenesis, regulating both transcription and processing of rRNA.

Original languageEnglish (US)
Article number10734
JournalNature communications
Volume7
DOIs
StatePublished - Feb 12 2016

Fingerprint

ribonucleic acids
Ribosomal RNA
proteins
Processing
Proteins
biological evolution
ribosomes
Ribosomes
18S Ribosomal RNA
Acetylation
acetylation
Cell proliferation
Transcription
NAD
cleavage
Cell Proliferation
Association reactions
attenuation
synthesis
U3 small nucleolar RNA

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing. / Chen, Sifan; Blank, Maximilian Felix; Iyer, Aishwarya; Huang, Bingding; Wang, Lin; Grummt, Ingrid; Voit, Renate.

In: Nature communications, Vol. 7, 10734, 12.02.2016.

Research output: Contribution to journalArticle

Chen, Sifan ; Blank, Maximilian Felix ; Iyer, Aishwarya ; Huang, Bingding ; Wang, Lin ; Grummt, Ingrid ; Voit, Renate. / SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing. In: Nature communications. 2016 ; Vol. 7.
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