Sodium butyrate with UCN-01 has marked antitumour activity against cervical cancer cells

Anne Zélie Decrion-Barthod, Matthieu Bosset, Marie Laure Plissonnier, Antonio Marchini, Magalie Nicolier, Sophie Launay, Jean Luc Prétet, Jean Rommelaere, Christiane Mougin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aim: The effect of combining sodium butyrate (NaB), a histone deacetylase inhibitor, and 7-hydroxy-staurosporine (UCN-01) on cytotoxicity in human cervical carcinoma cells was evaluated. Materials and Methods: HeLa and CaSki cells were treated using NaB alone or in combination with staurosporine (STS) or its analog UCN-01. Cytotoxicity was determined by flow cytometry and morphological assays. Apoptotic pathways were characterized by Western blotting and immunostaining. CaSki cells were also xenografted into nude mice to assess the in vivo effects of NaB/UCN-01 combination. Results: Treatment with NaB and STS or UCN-01 resulted in enhanced apoptosis of cancer cells. Apoptosis involved mitochondrial pathways and overexpression of p53 and p73. In concordance, co-treatment modulated some p53/p73 downstream targets such as p21, BAX, BCL-2 and BCL-XL, leading to increased caspase-3 and poly(ADP-ribose) polymerase cleavage. In vivo, NaB/UCN-01 combination exerted a substantial tumour growth suppression effect compared with single treatment. Conclusion: UCN-01 was shown to be a potentiator of NaB therapy for cervical cancer cells.

Original languageEnglish (US)
Pages (from-to)4049-4061
Number of pages13
JournalAnticancer research
Volume30
Issue number10
StatePublished - Oct 1 2010

Fingerprint

Butyric Acid
Uterine Cervical Neoplasms
Staurosporine
Apoptosis
Histone Deacetylase Inhibitors
Poly(ADP-ribose) Polymerases
7-hydroxystaurosporine
HeLa Cells
Nude Mice
Caspase 3
Neoplasms
Flow Cytometry
Western Blotting
Carcinoma
Growth

Keywords

  • Apoptosis
  • Cervical carcinoma
  • HPV
  • Sodium butyrate
  • UCN-01

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Decrion-Barthod, A. Z., Bosset, M., Plissonnier, M. L., Marchini, A., Nicolier, M., Launay, S., ... Mougin, C. (2010). Sodium butyrate with UCN-01 has marked antitumour activity against cervical cancer cells. Anticancer research, 30(10), 4049-4061.

Sodium butyrate with UCN-01 has marked antitumour activity against cervical cancer cells. / Decrion-Barthod, Anne Zélie; Bosset, Matthieu; Plissonnier, Marie Laure; Marchini, Antonio; Nicolier, Magalie; Launay, Sophie; Prétet, Jean Luc; Rommelaere, Jean; Mougin, Christiane.

In: Anticancer research, Vol. 30, No. 10, 01.10.2010, p. 4049-4061.

Research output: Contribution to journalArticle

Decrion-Barthod, AZ, Bosset, M, Plissonnier, ML, Marchini, A, Nicolier, M, Launay, S, Prétet, JL, Rommelaere, J & Mougin, C 2010, 'Sodium butyrate with UCN-01 has marked antitumour activity against cervical cancer cells', Anticancer research, vol. 30, no. 10, pp. 4049-4061.
Decrion-Barthod AZ, Bosset M, Plissonnier ML, Marchini A, Nicolier M, Launay S et al. Sodium butyrate with UCN-01 has marked antitumour activity against cervical cancer cells. Anticancer research. 2010 Oct 1;30(10):4049-4061.
Decrion-Barthod, Anne Zélie ; Bosset, Matthieu ; Plissonnier, Marie Laure ; Marchini, Antonio ; Nicolier, Magalie ; Launay, Sophie ; Prétet, Jean Luc ; Rommelaere, Jean ; Mougin, Christiane. / Sodium butyrate with UCN-01 has marked antitumour activity against cervical cancer cells. In: Anticancer research. 2010 ; Vol. 30, No. 10. pp. 4049-4061.
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AU - Nicolier, Magalie

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