Studying autophagic lysosome reformation in cells and by an in vitro reconstitution system

Yang Chen, Qian Peter Su, Li Yu

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Autophagic lysosome reformation (ALR) is the terminal step of autophagy. ALR functions to recycle lysosomal membranes and maintain lysosome homeostasis. Maintaining a functional lysosome pool is critical for generating autolysosomes, in which cellular components are degraded and turned over during autophagy. This unit describes methods to visualize ALR in cells. In addition, this unit provides detailed protocols to establish in vitro systems which can be used to reconstitute ALR as well as to reconstitute mitochondrial tubulation/network formation, another process that is driven by motor proteins.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages163-172
Number of pages10
DOIs
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1880
ISSN (Print)1064-3745

Keywords

  • Autolysosome
  • Autophagic lysosome reformation
  • Autophagy
  • Dynamic tubulation
  • In vitro reconstitution
  • Mitochondrial network formation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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