TY - JOUR
T1 - Substance P signaling contributes to granuloma formation in taenia crassiceps infection, a murine model of cysticercosis
AU - Garza, Armandina
AU - Tweardy, David J.
AU - Weinstock, Joel
AU - Viswanathan, Balaji
AU - Robinson, Prema
PY - 2010
Y1 - 2010
N2 - Cysticercosis is an infection with larval cysts of the cestode Taenia solium. Through pathways that are incompletely understood, dying parasites initiate a granulomatous reaction that, in the brain, causes seizures. Substance P (SP), a neuropeptide involved in pain-transmission, contributes to inflammation and previously was detected in granulomas associated with dead T. crassiceps cysts. To determine if SP contributes to granuloma formation, we measured granuloma-size and levels of IL-1β, TNF-α, and IL-6 within granulomas in T. crassiceps-infected wild type (WT)mice and mice deficient in SP-precursor (SPP) or the SP-receptor (neurokinin 1, NK1). Granuloma volumes of infected SPP-and NK1-knockout mice were reduced by 31 and 36%, respectively, compared to WT mice (P <.05 for both) and produced up to 5-fold less IL-1β, TNF-α, and IL-6 protein. Thus, SP signaling contributes to granuloma development and proinflammatory cytokine production in T. crassiceps infection and suggests a potential role for this mediator in human cystercercosis.
AB - Cysticercosis is an infection with larval cysts of the cestode Taenia solium. Through pathways that are incompletely understood, dying parasites initiate a granulomatous reaction that, in the brain, causes seizures. Substance P (SP), a neuropeptide involved in pain-transmission, contributes to inflammation and previously was detected in granulomas associated with dead T. crassiceps cysts. To determine if SP contributes to granuloma formation, we measured granuloma-size and levels of IL-1β, TNF-α, and IL-6 within granulomas in T. crassiceps-infected wild type (WT)mice and mice deficient in SP-precursor (SPP) or the SP-receptor (neurokinin 1, NK1). Granuloma volumes of infected SPP-and NK1-knockout mice were reduced by 31 and 36%, respectively, compared to WT mice (P <.05 for both) and produced up to 5-fold less IL-1β, TNF-α, and IL-6 protein. Thus, SP signaling contributes to granuloma development and proinflammatory cytokine production in T. crassiceps infection and suggests a potential role for this mediator in human cystercercosis.
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U2 - 10.1155/2010/597086
DO - 10.1155/2010/597086
M3 - Article
C2 - 20150970
AN - SCOPUS:77951920089
SN - 1110-7243
VL - 2010
JO - Journal of Biomedicine and Biotechnology
JF - Journal of Biomedicine and Biotechnology
M1 - 597086
ER -