99mTc-Hydroxydiphosphonate quantification of extracellular matrix mineralization in 3D human mesenchymal stem cell cultures

T. L. Grossner, Uwe Haberkorn, T. Gotterbarm

Research output: Contribution to journalArticle

Abstract

Objectives Bone tissue engineering is one of the fastest growing branches in modern bioscience. new methods are being developed to achieve higher grades of mineral deposition by osteogenically inducted mesenchymal stem cells. In addition to well established monolayer cell culture models, 3D cell cultures for stem cell-based osteogenic differentiation have become increasingly attractive to promote in vivo bone formation. one of the main problems of scaffold-based osteogenic cell cultures is the difficulty in quantifying the amount of newly produced extracellular mineral deposition, as a marker for new bone formation, without destroying the scaffold. In recent studies, we were able to show that 99mTc-methylene diphosphonate (99mTc-MDp), a gamma radiation-emitting radionuclide, can successfully be applied as a reliable quantitative marker for mineral deposition as this tracer binds with high affinity to newly produced hydroxyapatite (HA). Methods Within the present study, we evaluated whether this promising new method, using 99mTc-hydroxydiphosphonate (99mTc-HDp), can be used to quantify the amount of newly formed extracellular HA in a 3D cell culture model. Highly porous collagen type II scaffolds were seeded with 1 × 106 human mesenchymal stem cells (hMscs; n = 6) and cultured for 21 days in osteogenic media (group A – osteogenic (osM) group) and in parallel in standard media (group B – negative control (cnTRL) group). After incubation with 99mTc-HDp, the tracer uptake, reflected by the amount of emitted gamma counts, was measured. Results We saw a higher uptake (up to 15-fold) of the tracer in the osM group A compared with the cnTRL group B. statistical analysis of the results (student`s t-test) revealed a significantly higher amount of emitted gamma counts in the osM group (p = 0.048). Qualitative and semi-quantitative analysis by Alizarin Red staining confirmed the presence of extracellular HA deposition in the osM group. Conclusion our data indicate that 99mTc-HDp labelling is a promising tool to track and quantify nondestructive local HA deposition in 3D stem cell cultures.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalBone and Joint Research
Volume8
Issue number7
DOIs
StatePublished - Jan 1 2019

Fingerprint

Mesenchymal Stromal Cells
Extracellular Matrix
Durapatite
Cell Culture Techniques
Minerals
Osteogenesis
Stem Cells
Technetium Tc 99m Medronate
Control Groups
Collagen Type II
Gamma Rays
Tissue Engineering
Radioisotopes
Staining and Labeling
Students
Bone and Bones

Keywords

  • Hydroxylapatite quantification
  • Osteogenesis
  • Scaffold-based tissue repai
  • Technetium labeling

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

99mTc-Hydroxydiphosphonate quantification of extracellular matrix mineralization in 3D human mesenchymal stem cell cultures. / Grossner, T. L.; Haberkorn, Uwe; Gotterbarm, T.

In: Bone and Joint Research, Vol. 8, No. 7, 01.01.2019, p. 333-341.

Research output: Contribution to journalArticle

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