Suppression of gene expression of myeloperoxidase (MPO) by γ-interferon (IFN-γ) in HL60 cells

S. Kawano, E. Tatsumi, N. Yoneda, S. Nagata, N. Yamaguchi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The gene expression of myeloperoxidase (MPO) in HL60 cells, as tested by Northern blot analysis with a MPO gene probe, was markedly suppressed at 9 h after the addition of γ-interferon (IFN-γ) (200 U/ml), whereas cytochemically detected MPO activity, cell surface antigen expression, and cell morphology remained unchanged even at 48 h. IFN-γ of 50 U/ml was sufficient for the suppression at 24 h. When the HL60 cells treated with IFN- γ (200 U/ml) for 24 h were cultured in the absence of IFN-γ for another 24 h, the transcript of MPO gene reverted to a level comparable to that of the HL60 cells cultured thoroughly in the absence of IFN-γ, indicating the reversibility of the suppression. The suppression of MPO expression at RNA level, probably independent of differentiation, is a biological activity exerted by IFN-γ, which has not been previously reported.

Original languageEnglish (US)
Pages (from-to)81-85
Number of pages5
JournalLymphokine and Cytokine Research
Volume12
Issue number2
StatePublished - Jan 1 1993

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HL-60 Cells
Interferons
Peroxidase
Gene Expression
Surface Antigens
Northern Blotting
Genes
RNA

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Suppression of gene expression of myeloperoxidase (MPO) by γ-interferon (IFN-γ) in HL60 cells. / Kawano, S.; Tatsumi, E.; Yoneda, N.; Nagata, S.; Yamaguchi, N.

In: Lymphokine and Cytokine Research, Vol. 12, No. 2, 01.01.1993, p. 81-85.

Research output: Contribution to journalArticle

Kawano, S. ; Tatsumi, E. ; Yoneda, N. ; Nagata, S. ; Yamaguchi, N. / Suppression of gene expression of myeloperoxidase (MPO) by γ-interferon (IFN-γ) in HL60 cells. In: Lymphokine and Cytokine Research. 1993 ; Vol. 12, No. 2. pp. 81-85.
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