Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells

M. Fernanda Ledda; Soraya Adris; Alicia I. Bravo; Claudia Kairiyama; Laura Bover; Yuti Chernajovsky; Jose Mordoh; Osvaldo L. Podhajcer

doi:10.1038/nm0297-171

Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells

M. Fernanda Ledda, Soraya Adris, Alicia I. Bravo, Claudia Kairiyama, Laura Bover, Yuti Chernajovsky, Jose Mordoh, Osvaldo L. Podhajcer

Research output: Contribution to journal › Article › peer-review

210 Scopus citations

Abstract

Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development.

Original language	English (US)
Pages (from-to)	171-176
Number of pages	6
Journal	Nature medicine
Volume	3
Issue number	2
DOIs	https://doi.org/10.1038/nm0297-171
State	Published - 1997
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells",

abstract = "Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development.",

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T1 - Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells

AU - Fernanda Ledda, M.

AU - Adris, Soraya

AU - Bravo, Alicia I.

AU - Kairiyama, Claudia

AU - Bover, Laura

AU - Chernajovsky, Yuti

AU - Mordoh, Jose

AU - Podhajcer, Osvaldo L.

PY - 1997

Y1 - 1997

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AB - Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development.

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Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells

Abstract

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