Survival of Cloudman Mouse Melanoma Cells after Irradiation by Solar Wavelengths of Light

Krystyna Cieszka, Helene Z. Hill, Patrick Xin, Michael Azure, George J. Hill, Markus F. Meyenhofer, Raymond E. Boissy, David L. Mitchell

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A number of variants of Cloudman S91 mouse melanoma cells that differ with respect to the amount of pigment produced are available for study. In this report, we compare the photobiological responses of S91/amel, which contains about 1 pg of melanin per cell, with S91/I3, which contains about 3 pg/cell. Earlier studies had shown that UVC induced more oxidative damage (in the form of thymine glycols) in cell line S91/I3 than in S91/amel and that cell line S91/amel was more resistant to killing by UVC than S91/ 13. The present study finds that S91/amel cells are also relatively resistant to killing by near monochromatic UVB from a Philips TL01 fluorescent lamp and by near monochromatic UVA from a Philips HPW125 lamp. However, when the cells are irradiated with a Westinghouse FS20 polychromatic lamp, the S91/I3 cells are more resistant than the S91/amel cells. These findings cannot be explained on the basis of pigment induction because in S91/I3 this is about the same after UVB and FS20, although the maximum is reached earlier after UVB. Nor can our findings be explained on the basis of pyrimidine dimer formation, which is comparable in the two cell lines regardless of the type of irradiation. These results suggest that, with a pigment such as melanin, which absorbs light across the visible and ultraviolet ranges of the spectrum, cellular responses to monochromatic light do not necessarily predict responses to polychromatic mixtures.

Original languageEnglish (US)
Pages (from-to)193-200
Number of pages8
JournalPigment Cell Research
Volume10
Issue number4
DOIs
StatePublished - Aug 1997

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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    Cieszka, K., Hill, H. Z., Xin, P., Azure, M., Hill, G. J., Meyenhofer, M. F., Boissy, R. E., & Mitchell, D. L. (1997). Survival of Cloudman Mouse Melanoma Cells after Irradiation by Solar Wavelengths of Light. Pigment Cell Research, 10(4), 193-200. https://doi.org/10.1111/j.1600-0749.1997.tb00484.x