TY - JOUR
T1 - Survivin-targeted nanoparticles for pancreatic tumor imaging in mouse model
AU - Wang, Zhongqiu
AU - Tong, Mingmin
AU - Chen, Xiao
AU - Hu, Shouyou
AU - Yang, Zhijian
AU - Zhang, Yu
AU - Zhou, Hao
AU - Wu, Yuefei
AU - Li, Xiaoshuang
AU - Li, Donghui
N1 - Funding Information:
Grunt support: This study was supported by the National Natural Science Foundation of China (no.30870689, 81471705, 81271630) and Shanghai Municipal Science and Technology Commission (41902502).
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - We investigated the potential of targeting survivin, an inhibitor of apoptosis, in visualize pancreatic tumor in mouse model using targeted magnetic nanoparticles (MNPs) and magnetic resonance imaging (MRI). Chitosan-coated MNPS and survivin antisense oligonucleotide(ASON) were conjugated to give Sur-MNPs. Accumulations of targeted, non-targeted nanoparticles or nonsense oligonucleotide-MNPs (NSON-MNPs) in the liver, spleen, kidney and tumors were determined. Targeted nanoparticles were highly accumulated in BxPC-3 cells but not in non-cancer cells. In vivo MRI showed a significant T2 signal reduction in tumors of mice injected with targeted nanoparticles but slight signal change in tumors of mice injected with non-targeted nanoparticles or NSON-MNPs. Prussian blue staining demonstrated highly accumulated Sur-MNPs in tumor mass compared with normal pancreatic, kidney and liver tissues. Our data show that the MNPs functionalized with ASON lead to the targeted localization in pancreatic tumors. Survivin targeted nanoparticles could be used for detection of pancreatic tumors.
AB - We investigated the potential of targeting survivin, an inhibitor of apoptosis, in visualize pancreatic tumor in mouse model using targeted magnetic nanoparticles (MNPs) and magnetic resonance imaging (MRI). Chitosan-coated MNPS and survivin antisense oligonucleotide(ASON) were conjugated to give Sur-MNPs. Accumulations of targeted, non-targeted nanoparticles or nonsense oligonucleotide-MNPs (NSON-MNPs) in the liver, spleen, kidney and tumors were determined. Targeted nanoparticles were highly accumulated in BxPC-3 cells but not in non-cancer cells. In vivo MRI showed a significant T2 signal reduction in tumors of mice injected with targeted nanoparticles but slight signal change in tumors of mice injected with non-targeted nanoparticles or NSON-MNPs. Prussian blue staining demonstrated highly accumulated Sur-MNPs in tumor mass compared with normal pancreatic, kidney and liver tissues. Our data show that the MNPs functionalized with ASON lead to the targeted localization in pancreatic tumors. Survivin targeted nanoparticles could be used for detection of pancreatic tumors.
KW - Antisense oligonucleotide
KW - Magnetic nanoparticles
KW - Magnetic resonance imaging
KW - Pancreatic cancer
KW - Survivin
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U2 - 10.1016/j.nano.2016.02.008
DO - 10.1016/j.nano.2016.02.008
M3 - Article
C2 - 26995092
AN - SCOPUS:84966335590
SN - 1549-9634
VL - 12
SP - 1651
EP - 1661
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
IS - 6
ER -