T cell control of staphylococcal enterotoxin B (SEB) lethal sensitivity in mice: CD4+CD45RB(bright)/CD4+CD45RB(dim) balance defines susceptibility to SEB toxicity

Allon Canaan, Hadar Marcus, Tanya Burakova, Madga David, Benjamin Dekel, Harry Segall, Yair Reisner

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Radiation chimeras, generated by transplantation of SCID bone marrow into C3H/HeJ mice, show lethal susceptibility to staphylococcal enterotoxin B (SEB), thus constituting a valid murine model for SEB shock. This SEB sensitivity is due to the ability of the irradiated host to restore residual T cell populations, since the SCID donor bone marrow is unable to generate T cells. SCID bone marrow transplanted into irradiated nude mice does not generate SEB-sensitive chimeras, as a consequence of the inability of the recipient nude mice to develop mature T cells. Thymectomy of normal recipient mice prior to bone marrow transplantation does not affect the development of susceptibility to SEB, suggesting that post-thymic, residual T cells of the host probably mediate this SEB sensitivity. In vivo depletion experiments show that CD4+ T cells are required for the SEB-triggered shock, while CD8+ cells suppress it. A further examination of the T helper subpopulations in the SEB-sensitive mice reveals a prevalence of CD4+CD45RB(dim) cells over CD4+CD45RB(bright) cells. This T helper balance was statistically significant when correlated with SEB-induced mortality. Our model provides a possible explanation for the SEB resistance of normal mice: they have a prevalence of CD4+CD45RB(dim) over CD4+ CD45RB(bright) cells.

Original languageEnglish (US)
Pages (from-to)1375-1382
Number of pages8
JournalEuropean Journal of Immunology
Volume29
Issue number4
DOIs
StatePublished - Jan 1 1999

Keywords

  • CD45RB
  • Staphylococcal enterotoxin B
  • Superantigen
  • Th1
  • Th2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'T cell control of staphylococcal enterotoxin B (SEB) lethal sensitivity in mice: CD4<sup>+</sup>CD45RB(bright)/CD4<sup>+</sup>CD45RB(dim) balance defines susceptibility to SEB toxicity'. Together they form a unique fingerprint.

  • Cite this