Abstract
Signalling through the IFNαR (interferon-α receptor) and TCR (T-cell receptor) in Jurkat T lymphocytes results in distinct immune responses. Despite this both receptors elicit ERK (extracellular-signal-regulated kinase)/MAPK (mitogen-activated protein kinase) phosphorylation. Vav and Slp76 are shown to be required for IFNα (interferon-α)-stimulated ERK activity. These form a subset of proteins which behave identically on stimulation of both receptors. TCR deletion abrogates IFNα R-stimulated MAPK activity, whereas the canonical JAK/STAT (Janus kinase/signal transducer and activator of transcription) pathway is unaffected. Thus recruitment of the intact TCR ESC (early signalling complex) is necessary for this downstream MAPKresponse. Despite using a common ESC, stimulation of the IFNα R does not produce the transcriptional response associated with TCR. Up-regulation of the MAPK pathway by IFNα R might be important to ensure that the cell responds to only one stimulant.
Original language | English (US) |
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Pages (from-to) | 429-437 |
Number of pages | 9 |
Journal | Biochemical Journal |
Volume | 428 |
Issue number | 3 |
DOIs | |
State | Published - Jun 15 2010 |
Keywords
- Interferon-α receptor (IFNαR)
- Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT pathway)
- Mitogen-activated protein kinase pathway (MAPK pathway)
- Slp76
- T-cell receptor early signalling complex (TCR ESC)
- Vav
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology