T-cell receptor early signalling complex activation in response to interferon-α receptor stimulation

Claire N. Stevens, Ann Marie Simeone, Susan John, Zamal Ahmed, Orso M. Lucherini, C. Tatiana Baldari, John Edward Simon Durham Ladbury

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Signalling through the IFNαR (interferon-α receptor) and TCR (T-cell receptor) in Jurkat T lymphocytes results in distinct immune responses. Despite this both receptors elicit ERK (extracellular-signal-regulated kinase)/MAPK (mitogen-activated protein kinase) phosphorylation. Vav and Slp76 are shown to be required for IFNα (interferon-α)-stimulated ERK activity. These form a subset of proteins which behave identically on stimulation of both receptors. TCR deletion abrogates IFNα R-stimulated MAPK activity, whereas the canonical JAK/STAT (Janus kinase/signal transducer and activator of transcription) pathway is unaffected. Thus recruitment of the intact TCR ESC (early signalling complex) is necessary for this downstream MAPKresponse. Despite using a common ESC, stimulation of the IFNα R does not produce the transcriptional response associated with TCR. Up-regulation of the MAPK pathway by IFNα R might be important to ensure that the cell responds to only one stimulant.

Original languageEnglish (US)
Pages (from-to)429-437
Number of pages9
JournalBiochemical Journal
Volume428
Issue number3
DOIs
StatePublished - Jun 15 2010

Keywords

  • Interferon-α receptor (IFNαR)
  • Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT pathway)
  • Mitogen-activated protein kinase pathway (MAPK pathway)
  • Slp76
  • T-cell receptor early signalling complex (TCR ESC)
  • Vav

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'T-cell receptor early signalling complex activation in response to interferon-α receptor stimulation'. Together they form a unique fingerprint.

Cite this