TY - JOUR
T1 - Technologies for deriving primary tumor cells for use in personalized cancer therapy
AU - Mitra, Abhisek
AU - Mishra, Lopa
AU - Li, Shulin
N1 - Funding Information:
Work in the authors’ laboratory was supported by grants from the National Institutes of Health to Dr. Shulin Li (NIH R01CA120895) and Dr. Lopa Mishra (NIH 7P01CA130821). The University of Texas MD Anderson Cancer Center is supported in part by NCI CCSG Core Grant CA16672. Special thanks to Kaushik N. Thakkar, Srinivas Somanchi, Arun Satelli, Prasad Phatarpekar, Denada Dibra, and Dr. Reshmi Chatterjee for valuable suggestions and contributions towards this article.
PY - 2013/6
Y1 - 2013/6
N2 - For decades, immortal cancer cell lines have constituted an accessible, easily usable set of biological models to investigate cancer biology and explore the potential efficacy of anticancer drugs. However, numerous studies have suggested that these cell lines poorly represent the diversity, heterogeneity, and drug-resistant tumors occurring in patients. The derivation and short-term culture of primary cells from solid tumors have thus gained significant importance in personalized cancer therapy. This review focuses on our current understanding and the pros and cons of different methods for primary tumor cell culture. Furthermore, various culture matrices such as biomimetic scaffolds and chemically defined media supplemented with essential nutrients, have been prepared for different tissues. These well-characterized primary tumor cells redefine cancer therapies with high translational relevance.
AB - For decades, immortal cancer cell lines have constituted an accessible, easily usable set of biological models to investigate cancer biology and explore the potential efficacy of anticancer drugs. However, numerous studies have suggested that these cell lines poorly represent the diversity, heterogeneity, and drug-resistant tumors occurring in patients. The derivation and short-term culture of primary cells from solid tumors have thus gained significant importance in personalized cancer therapy. This review focuses on our current understanding and the pros and cons of different methods for primary tumor cell culture. Furthermore, various culture matrices such as biomimetic scaffolds and chemically defined media supplemented with essential nutrients, have been prepared for different tissues. These well-characterized primary tumor cells redefine cancer therapies with high translational relevance.
KW - Personalized therapy
KW - Primary tumor cell lines
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U2 - 10.1016/j.tibtech.2013.03.006
DO - 10.1016/j.tibtech.2013.03.006
M3 - Review article
C2 - 23597659
AN - SCOPUS:84878161769
SN - 0167-7799
VL - 31
SP - 347
EP - 354
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 6
ER -