Abstract
Hu et al. develop a protocol to analyze DNA-protein crosslinking (DPC) damage. Designated the ARK assay, this method outperforms widely used assays by allowing the detection of global DPCs with improved sensitivity and expanded readout. Defective DPC repair is detected in Fanconi anemia mutant cells by this protocol.
Original language | English (US) |
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Pages (from-to) | 1235-1245.e4 |
Journal | Cell Reports |
Volume | 30 |
Issue number | 4 |
DOIs | |
State | Published - Jan 28 2020 |
Keywords
- DNA damage response
- DNA-protein crosslinks
- DPC repair
- K-SDS
- RADAR
- camptothecin
- etoposide
- formaldehyde
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
MD Anderson CCSG core facilities
- Functional Genomics Core