The ARK Assay Is a Sensitive and Versatile Method for the Global Detection of DNA-Protein Crosslinks

Qianghua Hu, Naeh Klages-Mundt, Rui Wang, Erica Lynn, Liton Kuma Saha, Huimin Zhang, Mrinal Srivastava, Xi Shen, Yanyan Tian, Hyeung Kim, Yin Ye, Tanya Paull, Shunichi Takeda, Junjie Chen, Lei Li

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Hu et al. develop a protocol to analyze DNA-protein crosslinking (DPC) damage. Designated the ARK assay, this method outperforms widely used assays by allowing the detection of global DPCs with improved sensitivity and expanded readout. Defective DPC repair is detected in Fanconi anemia mutant cells by this protocol.

Original languageEnglish (US)
Pages (from-to)1235-1245.e4
JournalCell Reports
Volume30
Issue number4
DOIs
StatePublished - Jan 28 2020

Keywords

  • DNA damage response
  • DNA-protein crosslinks
  • DPC repair
  • K-SDS
  • RADAR
  • camptothecin
  • etoposide
  • formaldehyde

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

MD Anderson CCSG core facilities

  • Functional Genomics Core

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