The cell death pathway induced by metal halide complexes of pyridine and derivative ligands in hepatocellular carcinoma cells-necrosis or apoptosis

G. Kismali; F. M. Emen; T. Yesilkaynak; O. Meral; D. Demirkiran; T. Sel; N. Kulcu

The cell death pathway induced by metal halide complexes of pyridine and derivative ligands in hepatocellular carcinoma cells-necrosis or apoptosis

G. Kismali
, F. M. Emen
, T. Yesilkaynak
, O. Meral
, D. Demirkiran
, T. Sel
, N. Kulcu

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND, Drugs designed to restore programmed cell death might be effective against many cancer. It was aimed to study the possible apoptotic-necrotic effects of the pyridinehalide complexes such as dichlorodipyridinepalladium( II) (PdCl ₂L ¹ 2), dichlorodipyridinenickel(II) (NiCl ₂L ¹ 2), dichlorodipyridinecopper(II) (CuCl ₂L ¹ 2), dibromodipyridinecopper(II) (CuBr ₂L ¹ 2) and dichlorobis-(2,4-dimethylpyridine)copper(II) (CuCl ²L ₂ 2) in the hepatocarcinoma cells (Hep G2). METHODS, All complexes were characterized by elemental analysis, 1H-NMR, FT-IR and Far-IR spectroscopy. Apoptotic effects were evaluated by cell viability assay, DNA laddering assay, LDH assay, DAPI nuclear staining and caspase 1-3-9 activity analysis. RESULTS, According to cell proliferation/viability datas, CuCl ²L ₂ 2 was estimated the most toxic, NiCl ₂L ¹ 2 the least toxic complex. Treatment of CuCl ²L ₂ 2 in IC50 doses resulted in a remarkable increase lactate dehydrogenase, it was followed by CuBr ₂L ¹ 2 complex. Picnotic nuclei, anisonucleosis and nuclear condensations in 200 μM concentration of CuCl ²L ₂ 2 and CuCl ₂L ¹ 2 treated cells were observed with DAPI staining also DNA brakes were also determined with electrophoresis. Caspase 1, 3 and 9 increased activation were not detected. CONCLUSIONS, The present study results indicate that, PdCl ₂L ¹ 2, NiCl ₂L ¹ 2, CuCl ₂L ¹ 2, CuBr ₂L ¹ 2, Cu- Cl ²L ₂ 2 complexes have antiproliferative action on hepatocellular carcinoma cells. However it would be wrong to interpret this effect as an apoptosis or necrosis exactly.

Original language	English (US)
Pages (from-to)	1001-1012
Number of pages	12
Journal	European review for medical and pharmacological sciences
Volume	16
Issue number	8
State	Published - Aug 2012
Externally published	Yes

Keywords

Apoptosis
Cell death
Hepatocellular carcinoma
HepG
Metal halide complexes

ASJC Scopus subject areas

Pharmacology (medical)

Cite this

@article{7fac4d2310a94bec8f6735b03d47d401,

title = "The cell death pathway induced by metal halide complexes of pyridine and derivative ligands in hepatocellular carcinoma cells-necrosis or apoptosis",

abstract = "BACKGROUND, Drugs designed to restore programmed cell death might be effective against many cancer. It was aimed to study the possible apoptotic-necrotic effects of the pyridinehalide complexes such as dichlorodipyridinepalladium( II) (PdCl 2L 1 2), dichlorodipyridinenickel(II) (NiCl 2L 1 2), dichlorodipyridinecopper(II) (CuCl 2L 1 2), dibromodipyridinecopper(II) (CuBr 2L 1 2) and dichlorobis-(2,4-dimethylpyridine)copper(II) (CuCl 2L 2 2) in the hepatocarcinoma cells (Hep G2). METHODS, All complexes were characterized by elemental analysis, 1H-NMR, FT-IR and Far-IR spectroscopy. Apoptotic effects were evaluated by cell viability assay, DNA laddering assay, LDH assay, DAPI nuclear staining and caspase 1-3-9 activity analysis. RESULTS, According to cell proliferation/viability datas, CuCl 2L 2 2 was estimated the most toxic, NiCl 2L 1 2 the least toxic complex. Treatment of CuCl 2L 2 2 in IC50 doses resulted in a remarkable increase lactate dehydrogenase, it was followed by CuBr 2L 1 2 complex. Picnotic nuclei, anisonucleosis and nuclear condensations in 200 μM concentration of CuCl 2L 2 2 and CuCl 2L 1 2 treated cells were observed with DAPI staining also DNA brakes were also determined with electrophoresis. Caspase 1, 3 and 9 increased activation were not detected. CONCLUSIONS, The present study results indicate that, PdCl 2L 1 2, NiCl 2L 1 2, CuCl 2L 1 2, CuBr 2L 1 2, Cu- Cl 2L 2 2 complexes have antiproliferative action on hepatocellular carcinoma cells. However it would be wrong to interpret this effect as an apoptosis or necrosis exactly.",

keywords = "Apoptosis, Cell death, Hepatocellular carcinoma, HepG, Metal halide complexes",

author = "G. Kismali and Emen, \{F. M.\} and T. Yesilkaynak and O. Meral and D. Demirkiran and T. Sel and N. Kulcu",

year = "2012",

month = aug,

language = "English (US)",

volume = "16",

pages = "1001--1012",

journal = "European review for medical and pharmacological sciences",

issn = "1128-3602",

publisher = "Verduci Editore s.r.l",

number = "8",

}

TY - JOUR

T1 - The cell death pathway induced by metal halide complexes of pyridine and derivative ligands in hepatocellular carcinoma cells-necrosis or apoptosis

AU - Kismali, G.

AU - Emen, F. M.

AU - Yesilkaynak, T.

AU - Meral, O.

AU - Demirkiran, D.

AU - Sel, T.

AU - Kulcu, N.

PY - 2012/8

Y1 - 2012/8

N2 - BACKGROUND, Drugs designed to restore programmed cell death might be effective against many cancer. It was aimed to study the possible apoptotic-necrotic effects of the pyridinehalide complexes such as dichlorodipyridinepalladium( II) (PdCl 2L 1 2), dichlorodipyridinenickel(II) (NiCl 2L 1 2), dichlorodipyridinecopper(II) (CuCl 2L 1 2), dibromodipyridinecopper(II) (CuBr 2L 1 2) and dichlorobis-(2,4-dimethylpyridine)copper(II) (CuCl 2L 2 2) in the hepatocarcinoma cells (Hep G2). METHODS, All complexes were characterized by elemental analysis, 1H-NMR, FT-IR and Far-IR spectroscopy. Apoptotic effects were evaluated by cell viability assay, DNA laddering assay, LDH assay, DAPI nuclear staining and caspase 1-3-9 activity analysis. RESULTS, According to cell proliferation/viability datas, CuCl 2L 2 2 was estimated the most toxic, NiCl 2L 1 2 the least toxic complex. Treatment of CuCl 2L 2 2 in IC50 doses resulted in a remarkable increase lactate dehydrogenase, it was followed by CuBr 2L 1 2 complex. Picnotic nuclei, anisonucleosis and nuclear condensations in 200 μM concentration of CuCl 2L 2 2 and CuCl 2L 1 2 treated cells were observed with DAPI staining also DNA brakes were also determined with electrophoresis. Caspase 1, 3 and 9 increased activation were not detected. CONCLUSIONS, The present study results indicate that, PdCl 2L 1 2, NiCl 2L 1 2, CuCl 2L 1 2, CuBr 2L 1 2, Cu- Cl 2L 2 2 complexes have antiproliferative action on hepatocellular carcinoma cells. However it would be wrong to interpret this effect as an apoptosis or necrosis exactly.

AB - BACKGROUND, Drugs designed to restore programmed cell death might be effective against many cancer. It was aimed to study the possible apoptotic-necrotic effects of the pyridinehalide complexes such as dichlorodipyridinepalladium( II) (PdCl 2L 1 2), dichlorodipyridinenickel(II) (NiCl 2L 1 2), dichlorodipyridinecopper(II) (CuCl 2L 1 2), dibromodipyridinecopper(II) (CuBr 2L 1 2) and dichlorobis-(2,4-dimethylpyridine)copper(II) (CuCl 2L 2 2) in the hepatocarcinoma cells (Hep G2). METHODS, All complexes were characterized by elemental analysis, 1H-NMR, FT-IR and Far-IR spectroscopy. Apoptotic effects were evaluated by cell viability assay, DNA laddering assay, LDH assay, DAPI nuclear staining and caspase 1-3-9 activity analysis. RESULTS, According to cell proliferation/viability datas, CuCl 2L 2 2 was estimated the most toxic, NiCl 2L 1 2 the least toxic complex. Treatment of CuCl 2L 2 2 in IC50 doses resulted in a remarkable increase lactate dehydrogenase, it was followed by CuBr 2L 1 2 complex. Picnotic nuclei, anisonucleosis and nuclear condensations in 200 μM concentration of CuCl 2L 2 2 and CuCl 2L 1 2 treated cells were observed with DAPI staining also DNA brakes were also determined with electrophoresis. Caspase 1, 3 and 9 increased activation were not detected. CONCLUSIONS, The present study results indicate that, PdCl 2L 1 2, NiCl 2L 1 2, CuCl 2L 1 2, CuBr 2L 1 2, Cu- Cl 2L 2 2 complexes have antiproliferative action on hepatocellular carcinoma cells. However it would be wrong to interpret this effect as an apoptosis or necrosis exactly.

KW - Apoptosis

KW - Cell death

KW - Hepatocellular carcinoma

KW - HepG

KW - Metal halide complexes

UR - https://www.scopus.com/pages/publications/84865829173

UR - https://www.scopus.com/pages/publications/84865829173#tab=citedBy

M3 - Article

C2 - 22913148

AN - SCOPUS:84865829173

SN - 1128-3602

VL - 16

SP - 1001

EP - 1012

JO - European review for medical and pharmacological sciences

JF - European review for medical and pharmacological sciences

IS - 8

ER -

The cell death pathway induced by metal halide complexes of pyridine and derivative ligands in hepatocellular carcinoma cells-necrosis or apoptosis

Abstract

Keywords

ASJC Scopus subject areas

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