The cytotoxicity of lingshuiol: A comparative study with amphidinol 2 on membrane permeabilizing activities

Xin Ming Qi, Bo Yu, Xiao Chun Huang, Yue Wei Guo, Qing Zhai, Ren Jin

    Research output: Contribution to journalArticle

    5 Scopus citations

    Abstract

    The cytotoxicity of lingshuiol, a novel polyhydroxy compound with a linear carbon-chain isolated from the cultured marine dinoflagellate Amphidinium sp., and that of amphidinol 2 (AM2) was compared with hepatocytes. Both lingshuiol and AM2 were toxic to primary rat hepatocytes with IC50 values of 0.21 and 6.4 μM, respectively. Meanwhile, lingshuiol or AM2 caused a rapid mitochondrial swelling and leakage of Ca2+, underlying the change in permeability of mitochondria. Cyclosporin A, a specific inhibitor of mitochondrial permeability transition (MPT), could not affect these effects, indicating that CsA-sensitive MPT was not involved in the permeabilizing effects of lingshuiol or AM2. Sytox green tests further demonstrated that lingshuiol had a much stronger permeabilizing activity than AM2. Taken together, these results disclosed that lingshuiol had potent membrane permeabilizing activities, which might account for its cytotoxic effect.

    Original languageEnglish (US)
    Pages (from-to)278-282
    Number of pages5
    JournalToxicon
    Volume50
    Issue number2
    DOIs
    StatePublished - Aug 1 2007

    Keywords

    • Cytotoxicity
    • Lingshuiol
    • Marine
    • Membrane permeability
    • Polyhydroxy

    ASJC Scopus subject areas

    • Toxicology

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