The effect of chemotherapy on the number of treg in the peripheral blood of patients with breast cancer

Xiaoting Lu, Juntian Liu, Xiubao Ren, Hui Li, Jinpu Yu

    Research output: Contribution to journalArticle

    Abstract

    Objective: To investigate the effect of chemotherapy on Treg (CD4+CD25+ regulatory T cell) in the peripheral blood of patients with breast cancer who received radical mastectomy or modified radical mastectomy. Methods: A total of 52 samples of the peripheral blood of post-operated patients with breast cancer were collected on the 1st day before chemotherapy and the 10th day after chemotherapy. The proportion of Treg and CD3+, CD4+, and CD8+ T cells in the total amount of T cells was evaluated by flow cytometry. The cytokine such as IL-4, IL-10, TGF-β1 and IFN-γ were measured by ELLSA. Results: The proportion of Treg in the peripheral blood was smaller before chemotherapy than after chemotherapy (P<0.05). The changes in Treg was not correlated with lymph node status, the number of positive lymph nodes and menopause status(P>0.05). No statistical significance was found in the percentage of CD3+ and CD4+ T cells in the peripheral blood between the samples of pre- and post-chemotherapy (P>0.05). The proportion of CD8+ T cells in T lymphocytes increased from (24.876±6.631)% before chemotherapy to (28.129±10.900)% after chemotherapy(P<0.05), but CD4+/CD8+ decreased from (1.680±0.704)% to (1.506±0.691)%(P<0.05). The levels of IL-4, IL-10 and TGF-β1 after chemotherapy were lower than those before chemotherapy (P<0.05), but the level of 1FN-γ was higher after chemotherapy (P<0.05). However, the changes in Treg were not correlated with the changes in the level of cytokines such as IL-4, IL-10, TGF-β1 and IFN-γ. Conclusion: Chemotherapy may inhibit tumor through reducing Treg in the peripheral blood of patients with breast cancer.

    Original languageEnglish (US)
    Pages (from-to)508-511
    Number of pages4
    JournalChinese Journal of Clinical Oncology
    Volume35
    Issue number9
    StatePublished - Dec 1 2008

    Keywords

    • Breast cancer
    • Cd4+cd25+ regulatory t cell
    • Chemotherapy

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

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