The effect of high gravidity on the carcinogenesis of mammary gland in TA2 mice

Xuan Wang, Chun Huang, Baocun Sun, Yanjun Gu, Yanfen Cui, Xiulan Zhao, Yan Li, Shiwu Zhang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Problem: Spontaneous breast cancer in Tientsin Albinao 2 (TA2) mice, like human pregnancy-associated breast cancer (PABC), often occurs in pregnancy and puerperium, especially in mice with high gravidity. We hypothesized that the dysfunction of cellular immunity caused by the increase of 17β-estradiol (E2) and progesterone (P) might be one of the reasons for carcinogenesis of mammary gland. Method of study: We investigated the T lymphocyte subsets and the concentration of serum hormone and cytokines in cancer-bearing, pregnant or postpartum TA2 mice using flow cytometry, chemiluminescent immunoassay, and enzyme-linked immunosorbent assay (ELISA), respectively. Results: The number of T lymphocytes and the concentration of E2, P, interleukin-2 (IL-2), IL-4, and interferon-gamma (IFN-γ) changed with the increase of pregnancy and puerperium. During four pregnancies, elevated E2 and P resulted in a decrease in the number of CD3+, CD4+ T lymphocytes, CD4+/CD8+ ratio, and the concentration of IL-2, IL-4, and IFN-γ. Data in the fourth pregnancy were the closest to those of cancer-bearing mice. Conclusion: T lymphocyte subsets and concentration of IL-2, IL-4, and IFN-γ are affected by E2 and P during multiple pregnancy and delivery to some degree, which may contribute to the genesis of spontaneous breast cancer in TA2 mice.

Original languageEnglish (US)
Pages (from-to)396-409
Number of pages14
JournalAmerican Journal of Reproductive Immunology
Volume63
Issue number5
DOIs
StatePublished - May 1 2010

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Gravidity
Human Mammary Glands
Carcinogenesis
Pregnancy
Interleukin-4
Postpartum Period
Interferon-gamma
Interleukin-2
T-Lymphocyte Subsets
Breast Neoplasms
T-Lymphocytes
Multiple Pregnancy
CD4-CD8 Ratio
Immunoassay
Cellular Immunity
Progesterone
Estradiol
Neoplasms
Flow Cytometry
Enzyme-Linked Immunosorbent Assay

Keywords

  • Estradiol
  • Progesterone
  • Spontaneous breast cancer
  • T lymphocyte subsets
  • TA2 mice

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

The effect of high gravidity on the carcinogenesis of mammary gland in TA2 mice. / Wang, Xuan; Huang, Chun; Sun, Baocun; Gu, Yanjun; Cui, Yanfen; Zhao, Xiulan; Li, Yan; Zhang, Shiwu.

In: American Journal of Reproductive Immunology, Vol. 63, No. 5, 01.05.2010, p. 396-409.

Research output: Contribution to journalArticle

Wang, Xuan ; Huang, Chun ; Sun, Baocun ; Gu, Yanjun ; Cui, Yanfen ; Zhao, Xiulan ; Li, Yan ; Zhang, Shiwu. / The effect of high gravidity on the carcinogenesis of mammary gland in TA2 mice. In: American Journal of Reproductive Immunology. 2010 ; Vol. 63, No. 5. pp. 396-409.
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