The Escherichia coli ibpA thermometer is comprised of stable and unstable structural elements

Torsten Waldminghaus, Lena C. Gaubig, Birgit Klinkert, Franz Narberhaus

    Research output: Contribution to journalArticle

    35 Scopus citations

    Abstract

    Translation of many small heat shock genes in α- and γ-proteobacteria is controlled by the ROSE (Repression Of heat Shock gene Expression) element, a thermo-responsive RNA structure in the 5′-untranslated region. ROSEibpA regulates translation of the Escherichia coli ibpA gene coding for an inclusion body-associated protein. We present first structural insights into a full-length ROSE element by examining the temperature-induced conformational changes of ROSEibpA using detailed enzymatic and lead probing experiments between 20 and 50° C. The initial two hairpins are stable at all temperatures tested and might assist in proper folding of the third temperature-responsive stem-loop structure, which restricts access to the Shine-Dalgarno sequence at temperatures below 35° C. Toeprinting (primer extension inhibition) experiments show that binding of the 30S ribosome to ROSEibpA is enhanced at high temperatures. In contrast to other ROSE-like elements, the final hairpin is rather short. Single point mutations result in alternative structures with positive or negative effects on translation efficiency. Our study demonstrates how the combination of stable and unstable modules controls translation efficiency in a complete RNA thermometer.

    Original languageEnglish (US)
    JournalRNA Biology
    Volume6
    Issue number4
    DOIs
    StatePublished - Jan 1 2009

    Keywords

    • Heat shock
    • RNA structure
    • RNA thermometer
    • Riboswitch
    • Translational control

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

    Fingerprint Dive into the research topics of 'The Escherichia coli ibpA thermometer is comprised of stable and unstable structural elements'. Together they form a unique fingerprint.

  • Cite this