The induction and repair of (6-4) photoproducts in Neurospora crassa

T. I. Baker; R. J. Radloff; C. E. Cords; S. R. Engel; D. L. Mitchell

doi:10.1016/0921-8777(91)90024-J

The induction and repair of (6-4) photoproducts in Neurospora crassa

T. I. Baker
, R. J. Radloff
, C. E. Cords
, S. R. Engel
, D. L. Mitchell

Epigenetics & Molecular Carcinogenesis

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The (6-4) photoproduct lesion found in DNA after UV irradiations is repaired by germinating Neurospora crassa conidia. Wild-type Neurospora removes 80% of the (6-4) photoproduct in approximately 20 min and maximal repair is accomplished by 30 min with approximately 89% of the original lesions removed. Mutagen-sensitive Neurospora mutants belonging to the established excision repair epistasis group, UVS-2, are not defective in the removal of cyclobutane pyrimidine dimers. Furthermore, we find these mutants capable of removing (6-4) photoproducts from their DNA at a rate similar to wild type. Comparable kinetics are also observed in key members of the other two epistasis groups.

Original language	English (US)
Pages (from-to)	211-218
Number of pages	8
Journal	Mutation Research-DNA Repair
Volume	255
Issue number	3
DOIs	https://doi.org/10.1016/0921-8777(91)90024-J
State	Published - Nov 1991

Keywords

(6-4) Photoproducts
Neurospora crassa

ASJC Scopus subject areas

Molecular Biology
Toxicology
Genetics

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10.1016/0921-8777(91)90024-J

Cite this

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title = "The induction and repair of (6-4) photoproducts in Neurospora crassa",

abstract = "The (6-4) photoproduct lesion found in DNA after UV irradiations is repaired by germinating Neurospora crassa conidia. Wild-type Neurospora removes 80\% of the (6-4) photoproduct in approximately 20 min and maximal repair is accomplished by 30 min with approximately 89\% of the original lesions removed. Mutagen-sensitive Neurospora mutants belonging to the established excision repair epistasis group, UVS-2, are not defective in the removal of cyclobutane pyrimidine dimers. Furthermore, we find these mutants capable of removing (6-4) photoproducts from their DNA at a rate similar to wild type. Comparable kinetics are also observed in key members of the other two epistasis groups.",

keywords = "(6-4) Photoproducts, Neurospora crassa",

author = "Baker, \{T. I.\} and Radloff, \{R. J.\} and Cords, \{C. E.\} and Engel, \{S. R.\} and Mitchell, \{D. L.\}",

note = "Funding Information: This project was supported in part by the Dedicated Health Research Funds of the University of New Mexico School of Medicine and in part by DLM's appointment to the Alexander Hollaender Distinguished Postdoctoral Fellowship program sponsored by the U.S. Department of Energy, Office of Health and Environmental Research, administered by the Oak Ridge Associated Universities and DOE contract DE-AC03-76-SF01012.",

year = "1991",

month = nov,

doi = "10.1016/0921-8777(91)90024-J",

language = "English (US)",

volume = "255",

pages = "211--218",

journal = "Mutation Research-DNA Repair",

issn = "0921-8777",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - The induction and repair of (6-4) photoproducts in Neurospora crassa

AU - Baker, T. I.

AU - Radloff, R. J.

AU - Cords, C. E.

AU - Engel, S. R.

AU - Mitchell, D. L.

N1 - Funding Information: This project was supported in part by the Dedicated Health Research Funds of the University of New Mexico School of Medicine and in part by DLM's appointment to the Alexander Hollaender Distinguished Postdoctoral Fellowship program sponsored by the U.S. Department of Energy, Office of Health and Environmental Research, administered by the Oak Ridge Associated Universities and DOE contract DE-AC03-76-SF01012.

PY - 1991/11

Y1 - 1991/11

N2 - The (6-4) photoproduct lesion found in DNA after UV irradiations is repaired by germinating Neurospora crassa conidia. Wild-type Neurospora removes 80% of the (6-4) photoproduct in approximately 20 min and maximal repair is accomplished by 30 min with approximately 89% of the original lesions removed. Mutagen-sensitive Neurospora mutants belonging to the established excision repair epistasis group, UVS-2, are not defective in the removal of cyclobutane pyrimidine dimers. Furthermore, we find these mutants capable of removing (6-4) photoproducts from their DNA at a rate similar to wild type. Comparable kinetics are also observed in key members of the other two epistasis groups.

AB - The (6-4) photoproduct lesion found in DNA after UV irradiations is repaired by germinating Neurospora crassa conidia. Wild-type Neurospora removes 80% of the (6-4) photoproduct in approximately 20 min and maximal repair is accomplished by 30 min with approximately 89% of the original lesions removed. Mutagen-sensitive Neurospora mutants belonging to the established excision repair epistasis group, UVS-2, are not defective in the removal of cyclobutane pyrimidine dimers. Furthermore, we find these mutants capable of removing (6-4) photoproducts from their DNA at a rate similar to wild type. Comparable kinetics are also observed in key members of the other two epistasis groups.

KW - (6-4) Photoproducts

KW - Neurospora crassa

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UR - https://www.scopus.com/pages/publications/0026045996#tab=citedBy

U2 - 10.1016/0921-8777(91)90024-J

DO - 10.1016/0921-8777(91)90024-J

M3 - Article

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AN - SCOPUS:0026045996

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JO - Mutation Research-DNA Repair

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IS - 3

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The induction and repair of (6-4) photoproducts in Neurospora crassa

Abstract

Keywords

ASJC Scopus subject areas

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