The metabolism and binding of catecholamines by the hepatic microsomal mixed function oxidase of the rat

Noradrenaline and adrenaline were metabolized by an NADPH and oxygen dependent process located within the hepatic microsomal fraction of the rat. Metabolism was inhibited by CO and compound SKF 525A, but not by pargyline, an inhibitor of monoamine oxidase, or by 3,4 dimethoxy 5 hydroxybenzoic acid, an inhibitor of catechol O methyltransferase. It is concluded that the enzyme system responsible for the metabolism of the catecholamines was the microsomal mixed function oxidase. The Km for noradrenaline was 2.4 mM and for adrenaline 1.0 mM, and V15.6 and 3.6 nmol/min per mg of microsomal protein respectively. Both catecholamines bound to the microsomal fraction, producing a type II spectral change, with a Ks for noradrenaline of 0.9 mM and for adrenaline of 1.0 mM, and showed other characteristics of type II compounds by inhibiting the reduction of cytochrome P 450 by NADPH and exhibiting an enhanced metabolism in the presence of acetone. The major product of catecholamine metabolism was an as yet unidentified alkali labile compound, which did not correspond to any of the recognized catecholamine metabolites.