TY - JOUR
T1 - The use of promoter fusions in Drosophila genetics
T2 - Isolation of mutations affecting the heat shock response
AU - Jose Bonner, J.
AU - Parks, Carol
AU - Parker-Thornburg, Janice
AU - Mortin, Mark A.
AU - Pelham, Hugh R.B.
N1 - Funding Information:
This work was supported by grant #GM26693 from the National Institutes of Health. J. J. B. was supported by the American Cancer Society Junior Faculty Research Award JFRA 58.
PY - 1984/7
Y1 - 1984/7
N2 - We have constructed a gene fusion using the promoter of Drosophila hsp70 and the structural gene for Drosophila alcohol dehydrogenase (Adh) and used this construct to transform Adh-deficient flies. In these transformants, Adh is expressed only after heat shock. Like hsp70 itself, this heat-shock-inducible Adh (Adhhs) is induced in a wide variety of tissues. It fails to be induced in primary spermatocytes. Although the tissue distribution of Adh activity is very different from wild type, this does not appear to be deleterious. Indeed, the induction of Adhhs allows flies to survive exposure to ethanol. We have used this latter characteristic to select dominant, trans-acting mutations that alter the response of flies to heat shock.
AB - We have constructed a gene fusion using the promoter of Drosophila hsp70 and the structural gene for Drosophila alcohol dehydrogenase (Adh) and used this construct to transform Adh-deficient flies. In these transformants, Adh is expressed only after heat shock. Like hsp70 itself, this heat-shock-inducible Adh (Adhhs) is induced in a wide variety of tissues. It fails to be induced in primary spermatocytes. Although the tissue distribution of Adh activity is very different from wild type, this does not appear to be deleterious. Indeed, the induction of Adhhs allows flies to survive exposure to ethanol. We have used this latter characteristic to select dominant, trans-acting mutations that alter the response of flies to heat shock.
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U2 - 10.1016/0092-8674(84)90432-X
DO - 10.1016/0092-8674(84)90432-X
M3 - Article
C2 - 6430570
AN - SCOPUS:0021698711
SN - 0092-8674
VL - 37
SP - 979
EP - 991
JO - Cell
JF - Cell
IS - 3
ER -