@article{21f92bd1b8b54a1e92f7f75e6239ad26,
title = "Thiol profiling in cancer cell lines by HPLC-mass spectrometry",
abstract = "We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Koppula et al. (2021).",
keywords = "Cancer, Mass Spectrometry, Metabolism",
author = "Xiaoguang Liu and Pranavi Koppula and Kellen Olszewski and Boyi Gan",
note = "Funding Information: This research was supported by Institutional Research Fund and Bridge Fund from The University of Texas MD Anderson Cancer Center, Emerson Collective Cancer Research Fund and R01CA181196, R01CA244144, and R01CA247992 from the National Institutes of Health (to B.G.). B.G. was an Andrew Sabin Family Fellow. P.K. was supported by CPRIT Research Training Grant (RP170067) and the Dr. John J. Kopchick Research Award from The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences. This research has also been supported by the National Institutes of Health Cancer Center Support Grant P30CA016672 to The University of Texas MD Anderson Cancer Center. P.K. X.L. K.O. and B.G. organized the protocol design. P.K. X.L. and B.G. wrote the sample preparation protocol. K.O. wrote the HPLC-MS method protocol for thiol analysis. B.G. initiated the manuscript and provided funding support for the research described in this protocol. K.O. is a full-time employee of the Barer Institute. The other authors declare no competing interests. Funding Information: This research was supported by Institutional Research Fund and Bridge Fund from The University of Texas MD Anderson Cancer Center , Emerson Collective Cancer Research Fund and R01CA181196 , R01CA244144 , and R01CA247992 from the National Institutes of Health (to B.G.). B.G. was an Andrew Sabin Family Fellow. P.K. was supported by CPRIT Research Training Grant ( RP170067 ) and the Dr. John J. Kopchick Research Award from The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences . This research has also been supported by the National Institutes of Health Cancer Center Support Grant P30CA016672 to The University of Texas MD Anderson Cancer Center . Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = dec,
day = "17",
doi = "10.1016/j.xpro.2021.100977",
language = "English (US)",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",
}