Transcriptional Scaffold: CIITA interacts with NF-Y, RFX, and CREB to cause stereospecific regulation of the Class II Major histocompatibility complex promoter

X. S. Zhu, M. W. Linhoff, G. Li, K. C. Chin, S. N. Maity, J. P.Y. Ting

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Abstract

Scaffold molecules interact with multiple effectors to elicit specific signal transduction pathways. CIITA, a non-DNA-binding regulator of class II major histocompatibility complex (MHC) gene transcription, may serve as a transcriptional scaffold. Regulation of the class II MHC promoter by CIITA requires strict spatial-helical arrangements of the X and Y promoter elements. The X element binds RFX (RFX5/RFXANK-RFXB/RFXAP) and CREB, while Y binds NF-Y/CBF (NF-YA, NF-YB, and NF-YC). CIITA interacts with all three. In vivo analysis using both N-terminal and C-terminal deletion constructs identified critical domains of CIITA that are required for interaction with NF-YB, NF-YC, RFX5, RFXANK/RFXB, and CREB. We propose that binding of NF-Y/CBF, RFX, and CREB by CIITA results in a macromolecular complex which allows transcription factors to interact with the class II MHC promoter in a spatially and helically constrained fashion.

Original languageEnglish (US)
Pages (from-to)6051-6061
Number of pages11
JournalMolecular and cellular biology
Volume20
Issue number16
DOIs
StatePublished - Aug 21 2000

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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