Transforming growth factor-alpha secretion by epidermal growth factor-dependent human tumor cell lines

S. E. Singletary, N. N. Williams, U. Rodeck, L. Larry, Susan L Tucker, G. Spitzer, M. Herlyn

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Pairs of cell lines from spontaneous human tumors (cervical adenocarcinoma, melanoma, and synovial sarcoma) were established using serum-free culture conditions with and without exogenous epidermal growth factor factor (EGF). EGF-adapted cultures of melanoma and cervical adenocarcinoma origin secreted higher levels of bioactive transforming growth factor alpha (TGF-α) when compared to cultures maintained in the absence of EGF. Depletion of EGF for these EGF-adapted cultures resulted in growth arrest. In contrast, the sarcoma cell lines did not secrete TGF-α regardless of the culture conditions but EGF significantly stimulated proliferation of these cells in short-term assays. We show that exogenous EGF induces TGF-α production and supports proliferation of tumor cells of various tissue origin but is not essential for in vitro growth factor-deprived conditions.

Original languageEnglish (US)
Pages (from-to)1501-1505
Number of pages5
JournalAnticancer research
Volume10
Issue number6
StatePublished - Dec 1 1990

Keywords

  • epidermal growth factor
  • human tumors
  • serum-free medium
  • transforming growth factor-α

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Singletary, S. E., Williams, N. N., Rodeck, U., Larry, L., Tucker, S. L., Spitzer, G., & Herlyn, M. (1990). Transforming growth factor-alpha secretion by epidermal growth factor-dependent human tumor cell lines. Anticancer research, 10(6), 1501-1505.