Transgenic mice express the human phenylethanolamine N-methyltransferase gene in adrenal medulla and retina

E. E. Baetge, Richard R Behringer, A. Messing, R. L. Brinster, R. D. Palmiter

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Abstract

The human gene for phenylethanolamine N-methyltransferase (hPNMT), responsible for the conversion of norepinephrine to epinephrine, has been cloned and the complete nucleotide sequence has been determined. The structural gene consists of three axons and two introns spanning ~2100 base pairs. Transgenic mice containing the hPNMT gene with either 2 or 8 kilobases of 5'-flanking sequence were produced and resulted in expression of hPNMT mRNA in the adrenal gland and eye. A chimeric gene consisting of 2 kilobases of the hPNMT 5'-flanking region fused to the simian virus 40 early region also resulted in tumor (T) antigen mRNA expression in adrenal glands and eyes; furthermore, immunocytochemistry showed that tumor antigen was localized in nuclei of adrenal medullary cells and cells of the inner nuclear cell layer of the retina, prominent sites of epinephrine synthesis. These results indicate that the enhancer(s) for appropriate expression of the hPNMT gene in these cell types is in the 2-kilobase 5'-flanking region of the human gene.

Original languageEnglish (US)
Pages (from-to)3648-3652
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number10
DOIs
StatePublished - Jan 1 1988

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Phenylethanolamine N-Methyltransferase
Adrenal Medulla
Transgenic Mice
Retina
Genes
5' Flanking Region
Neoplasm Antigens
Adrenal Glands
Epinephrine
Gene Expression
Messenger RNA
Simian virus 40
Viral Tumor Antigens
Base Pairing
Introns
Axons
Norepinephrine
Immunohistochemistry

ASJC Scopus subject areas

  • General

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Transgenic mice express the human phenylethanolamine N-methyltransferase gene in adrenal medulla and retina. / Baetge, E. E.; Behringer, Richard R; Messing, A.; Brinster, R. L.; Palmiter, R. D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 10, 01.01.1988, p. 3648-3652.

Research output: Contribution to journalArticle

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