Translation of Satellite Tobacco Necrosis Virus Ribonucleic Acid. I. Characterization of in Vitro Procaryotic and Eucaryotic Translation Products

William H. Klein; Chris Nolan; Jerome M. Lazar; John M. Clark

doi:10.1021/bi00761a003

Translation of Satellite Tobacco Necrosis Virus Ribonucleic Acid. I. Characterization of in Vitro Procaryotic and Eucaryotic Translation Products

William H. Klein, Chris Nolan, Jerome M. Lazar, John M. Clark

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

The in vitro translation of satellite tobacco necrosis virus RNA can be carried out by procaryotic (Escherichia coli) and eucaryotic (wheat embryo) systems. Fingerprint analyses reveal that both in vitro systems produce protein with the amino acid sequence of satellite tobacco necrosis virus coat protein. Gel filtration and acrylamide gel electrophoretic analyses show that both in vitro systems produce a single material with an average size slightly smaller than satellite tobacco necrosis virus coat protein.

Original language	English (US)
Pages (from-to)	2009-2014
Number of pages	6
Journal	Biochemistry
Volume	11
Issue number	11
DOIs	https://doi.org/10.1021/bi00761a003
State	Published - May 1 1972

ASJC Scopus subject areas

Biochemistry

Access to Document

10.1021/bi00761a003

Cite this

@article{ad16e87246a1426db0f4c708f4ab5493,

title = "Translation of Satellite Tobacco Necrosis Virus Ribonucleic Acid. I. Characterization of in Vitro Procaryotic and Eucaryotic Translation Products",

abstract = "The in vitro translation of satellite tobacco necrosis virus RNA can be carried out by procaryotic (Escherichia coli) and eucaryotic (wheat embryo) systems. Fingerprint analyses reveal that both in vitro systems produce protein with the amino acid sequence of satellite tobacco necrosis virus coat protein. Gel filtration and acrylamide gel electrophoretic analyses show that both in vitro systems produce a single material with an average size slightly smaller than satellite tobacco necrosis virus coat protein.",

author = "Klein, {William H.} and Chris Nolan and Lazar, {Jerome M.} and Clark, {John M.}",

year = "1972",

month = may,

day = "1",

doi = "10.1021/bi00761a003",

language = "English (US)",

volume = "11",

pages = "2009--2014",

journal = "Biochemistry",

issn = "0006-2960",

publisher = "American Chemical Society",

number = "11",

}

TY - JOUR

T1 - Translation of Satellite Tobacco Necrosis Virus Ribonucleic Acid. I. Characterization of in Vitro Procaryotic and Eucaryotic Translation Products

AU - Klein, William H.

AU - Nolan, Chris

AU - Lazar, Jerome M.

AU - Clark, John M.

PY - 1972/5/1

Y1 - 1972/5/1

N2 - The in vitro translation of satellite tobacco necrosis virus RNA can be carried out by procaryotic (Escherichia coli) and eucaryotic (wheat embryo) systems. Fingerprint analyses reveal that both in vitro systems produce protein with the amino acid sequence of satellite tobacco necrosis virus coat protein. Gel filtration and acrylamide gel electrophoretic analyses show that both in vitro systems produce a single material with an average size slightly smaller than satellite tobacco necrosis virus coat protein.

AB - The in vitro translation of satellite tobacco necrosis virus RNA can be carried out by procaryotic (Escherichia coli) and eucaryotic (wheat embryo) systems. Fingerprint analyses reveal that both in vitro systems produce protein with the amino acid sequence of satellite tobacco necrosis virus coat protein. Gel filtration and acrylamide gel electrophoretic analyses show that both in vitro systems produce a single material with an average size slightly smaller than satellite tobacco necrosis virus coat protein.

UR - http://www.scopus.com/inward/record.url?scp=0015520556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015520556&partnerID=8YFLogxK

U2 - 10.1021/bi00761a003

DO - 10.1021/bi00761a003

M3 - Article

C2 - 4554894

AN - SCOPUS:0015520556

SN - 0006-2960

VL - 11

SP - 2009

EP - 2014

JO - Biochemistry

JF - Biochemistry

IS - 11

ER -

Translation of Satellite Tobacco Necrosis Virus Ribonucleic Acid. I. Characterization of in Vitro Procaryotic and Eucaryotic Translation Products

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this