Tsp66E, the Drosophila KAI1 homologue, and Tsp74F function to regulate ovarian follicle cell and wing development by stabilizing integrin localization

Seung Yeop Han; Minjung Lee; Yoon Ki Hong; Soojin Hwang; Gahee Choi; Yoon Seok Suh; Seung Hwan Park; Soojin Lee; Sang Hee Lee; Jongkyeong Chung; Sung Hee Baek; Kyoung Sang Cho

doi:10.1016/j.febslet.2012.09.044

Tsp66E, the Drosophila KAI1 homologue, and Tsp74F function to regulate ovarian follicle cell and wing development by stabilizing integrin localization

Seung Yeop Han, Minjung Lee, Yoon Ki Hong, Soojin Hwang, Gahee Choi, Yoon Seok Suh, Seung Hwan Park, Soojin Lee, Sang Hee Lee, Jongkyeong Chung, Sung Hee Baek, Kyoung Sang Cho

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The metastasis suppressor KAI1/CD82 has been implicated in various cellular processes; however, its function in development is not fully understood. Here, we generated and characterized mutants of Tsp66E and Tsp74F, which are Drosophila homologues of KAI1/CD82 and Tspan11, respectively. These mutants exhibited egg elongation defects along with disturbed integrin localization and actin polarity. Moreover, the defects were enhanced by mutation of inflated, an αPS2 integrin gene. Mutant ovaries had elevated αPS2 integrin levels and reduced endocytic trafficking. These results suggest that Drosophila KAI1/CD82 affects the polarized localization and the level of integrin, which may contribute to epithelial cell polarity.

Original language	English (US)
Pages (from-to)	4031-4037
Number of pages	7
Journal	FEBS Letters
Volume	586
Issue number	22
DOIs	https://doi.org/10.1016/j.febslet.2012.09.044
State	Published - Nov 16 2012
Externally published	Yes

Keywords

Drosophila
Integrin
KAI1/CD82
Tsp66E
Tsp74F
Tspan11

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2012.09.044

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Han, S. Y., Lee, M., Hong, Y. K., Hwang, S., Choi, G., Suh, Y. S., Park, S. H., Lee, S., Lee, S. H., Chung, J., Baek, S. H., & Cho, K. S. (2012). Tsp66E, the Drosophila KAI1 homologue, and Tsp74F function to regulate ovarian follicle cell and wing development by stabilizing integrin localization. FEBS Letters, 586(22), 4031-4037. https://doi.org/10.1016/j.febslet.2012.09.044

@article{0db4ba8b6c1c4b2e8b3404e3422c0454,

title = "Tsp66E, the Drosophila KAI1 homologue, and Tsp74F function to regulate ovarian follicle cell and wing development by stabilizing integrin localization",

abstract = "The metastasis suppressor KAI1/CD82 has been implicated in various cellular processes; however, its function in development is not fully understood. Here, we generated and characterized mutants of Tsp66E and Tsp74F, which are Drosophila homologues of KAI1/CD82 and Tspan11, respectively. These mutants exhibited egg elongation defects along with disturbed integrin localization and actin polarity. Moreover, the defects were enhanced by mutation of inflated, an αPS2 integrin gene. Mutant ovaries had elevated αPS2 integrin levels and reduced endocytic trafficking. These results suggest that Drosophila KAI1/CD82 affects the polarized localization and the level of integrin, which may contribute to epithelial cell polarity.",

keywords = "Drosophila, Integrin, KAI1/CD82, Tsp66E, Tsp74F, Tspan11",

author = "Han, {Seung Yeop} and Minjung Lee and Hong, {Yoon Ki} and Soojin Hwang and Gahee Choi and Suh, {Yoon Seok} and Park, {Seung Hwan} and Soojin Lee and Lee, {Sang Hee} and Jongkyeong Chung and Baek, {Sung Hee} and Cho, {Kyoung Sang}",

note = "Funding Information: This work was supported by Grants from the Korea Research Foundation funded by the Korean Government ( KRF-2008-331-1-C00229 and KOSEF-2003-214-1-C00097 ). ",

year = "2012",

month = nov,

day = "16",

doi = "10.1016/j.febslet.2012.09.044",

language = "English (US)",

volume = "586",

pages = "4031--4037",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "22",

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T1 - Tsp66E, the Drosophila KAI1 homologue, and Tsp74F function to regulate ovarian follicle cell and wing development by stabilizing integrin localization

AU - Han, Seung Yeop

AU - Lee, Minjung

AU - Hong, Yoon Ki

AU - Hwang, Soojin

AU - Choi, Gahee

AU - Suh, Yoon Seok

AU - Park, Seung Hwan

AU - Lee, Soojin

AU - Lee, Sang Hee

AU - Chung, Jongkyeong

AU - Baek, Sung Hee

AU - Cho, Kyoung Sang

N1 - Funding Information: This work was supported by Grants from the Korea Research Foundation funded by the Korean Government ( KRF-2008-331-1-C00229 and KOSEF-2003-214-1-C00097 ).

PY - 2012/11/16

Y1 - 2012/11/16

N2 - The metastasis suppressor KAI1/CD82 has been implicated in various cellular processes; however, its function in development is not fully understood. Here, we generated and characterized mutants of Tsp66E and Tsp74F, which are Drosophila homologues of KAI1/CD82 and Tspan11, respectively. These mutants exhibited egg elongation defects along with disturbed integrin localization and actin polarity. Moreover, the defects were enhanced by mutation of inflated, an αPS2 integrin gene. Mutant ovaries had elevated αPS2 integrin levels and reduced endocytic trafficking. These results suggest that Drosophila KAI1/CD82 affects the polarized localization and the level of integrin, which may contribute to epithelial cell polarity.

AB - The metastasis suppressor KAI1/CD82 has been implicated in various cellular processes; however, its function in development is not fully understood. Here, we generated and characterized mutants of Tsp66E and Tsp74F, which are Drosophila homologues of KAI1/CD82 and Tspan11, respectively. These mutants exhibited egg elongation defects along with disturbed integrin localization and actin polarity. Moreover, the defects were enhanced by mutation of inflated, an αPS2 integrin gene. Mutant ovaries had elevated αPS2 integrin levels and reduced endocytic trafficking. These results suggest that Drosophila KAI1/CD82 affects the polarized localization and the level of integrin, which may contribute to epithelial cell polarity.

KW - Drosophila

KW - Integrin

KW - KAI1/CD82

KW - Tsp66E

KW - Tsp74F

KW - Tspan11

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SN - 0014-5793

VL - 586

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JO - FEBS Letters

JF - FEBS Letters

IS - 22

ER -

Tsp66E, the Drosophila KAI1 homologue, and Tsp74F function to regulate ovarian follicle cell and wing development by stabilizing integrin localization

Abstract

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