TT2016 meeting report on the 13th Transgenic Technology meeting in Prague, Czech Republic

TY - JOUR

T1 - TT2016 meeting report on the 13th Transgenic Technology meeting in Prague, Czech Republic

AU - Tamowski, Susan

AU - Luo, Jinping

AU - Kanzler, Benoît

AU - Whitelaw, Bruce

AU - Crispo, Martina

AU - Doglio, Lynn

AU - Jerchow, Boris

AU - Parker-Thornburg, Jan

N1 - Funding Information: On the last day, the ISTT Young Investigator Award (sponsored by inGenious Targeting Laboratory) was presented as well as the Best Poster Awards. The ISTT Young Investigator Award was given to Pablo Ross (University of California Davis, Davis, USA) based on his work developing ES cells in farm animals. He was trying to engineer interspecies chimeric blastocysts towards making use of pigs to grow human organs. The Best Poster Awards were selected by a committee of ISTT members who chose from more than 125 posters that were displayed throughout the meeting in the spacious poster room. Three posters were chosen as Poster Award winners, including Vera Jansen (Optogenetic Tools To Study Camp Signaling In Cilia And Flagella), Charles-Etienne Dumeau (Efficient Method For The Isolation Of Functional Single Cell From The ICM Of Mouse Blastocyst), and Hiromi Miura (Generation of Knockdown Mice by CRISPR/Cas9-based Targeted Insertion of Artificial miRNA Sequence). The winners received a beautiful Bohemian glass vase handmade in the Czech Republic. Funding Information: In session 9, titled “Gene Manipulation and Genome Editing and (disease) Models I, we had two stimulating talks on how CRISPR/Cas9 is being used both in “active genetics” (i.e. gene drive) and to induce deleterious mutations. Valentino Gantz (University of California San Diego, La Jolla, USA) discussed his recent work in establishing gene drive mechanisms in Drosophila essentially causing a mutagenic chain reaction and how this could be utilized to reduce/eliminate disease-causing insects. This was followed by a talk by Didier Stanier (Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany) who used CRISPR/Cas9 to induce mutations in zebrafish that compromised the Egf17 gene and found that total gene knockout would result in genetic compensation whereas knockdowns by morpholinos do not. This has implications for mutant models of diseases that are caused by mutation rather than by reduction of protein product. Session 10, a continuation of the Disease Models topics, started off with a presentation from Tania Sorg-Guss (CERMB-BIE, Illkirch, France) who was standing in for Yann Herault (PHENOMIN, Illkirch, France). She described GENCODYS which is a research consortium funded by the European Union dedicated to discover the functions and dysfunctions of the brain. They are using next generation sequencing to identify genes associated with low IQ in humans using mouse models. Katherina Boroviak (The Welcome Trust Sanger Institute, Cambridge, UK) discussed the limitations and possibilities of CRISPR/Cas technology including large chromosomal rearrangements and small insertions via cytoplasmic injection, resulting in inversions, duplications and point mutations. Kevin Peterson (The Jackson Labs, Bar Harbor, USA) described using CRISPR/Cas mediated gene modification to generate mouse models of human developmental disorders.

PY - 2016/8/1

Y1 - 2016/8/1

UR - http://www.scopus.com/inward/record.url?scp=84976634457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976634457&partnerID=8YFLogxK

U2 - 10.1007/s11248-016-9966-0

DO - 10.1007/s11248-016-9966-0

M3 - Article

C2 - 27277423

AN - SCOPUS:84976634457

SN - 0962-8819

VL - 25

SP - 553

EP - 559

JO - Transgenic Research

JF - Transgenic Research

IS - 4

ER -