TY - JOUR
T1 - Tyrosine phosphorylation of platelet derived growth factor β receptors in coronary artery lesions
T2 - Implications for vascular remodelling after directional coronary atherectomy and unstable angina pectoris
AU - Abe, J.
AU - Deguchi, J.
AU - Takuwa, Y.
AU - Hara, K.
AU - Ikari, Y.
AU - Tamura, T.
AU - Ohno, M.
AU - Kurokawa, K.
PY - 1998
Y1 - 1998
N2 - Background - Growth factors such as platelet derived growth factor (PDGF) have been postulated to be important mediators of neointimal proliferation observed in atherosclerotic plaques and restenotic lesions following coronary interventions. Binding of PDGF to its receptor results in intrinsic receptor tyrosine kinase activation and subsequent cellular migration, proliferation, and vascular contraction. Aims - To investigate whether the concentration of PDGF β receptor tyrosine phosphorylation obtained from directional coronary atherectomy (DCA) samples correlate with atherosclerotic plaque burden, the ability of diseased vessels to remodel, coronary risk factors, and clinical events. Methods - DCA samples from 59 patients and 15 non-atherosclerotic left internal thoracic arteries (LITA) were analysed for PDGF β receptor tyrosine phosphorylation content by receptor immunoprecipitation and antiphosphotyrosine western blot. The amount of PDGF β receptor phosphorylation was analysed in relation to angiographic follow up data and clinical variables. Results - PDGF β receptor tyrosine phosphorylation in the 59 DCA samples was greater than in the 15 non- atherosclerotic LITA (mean (SD) 0.84 (0.67) υ 0.17 (0.08) over a control standard, p < 0.0001). As evaluated by stepwise regression analysis, incorporation of both PDGF β receptor tyrosine phosphorylation and immediate gain correlated strongly (adjusted r2 = 0.579) with late loss, although PDGF β receptor tyramine phosphorylation alone correlated poorly with late loss. Multivariate regression analysis of coronary risk factors and clinical events revealed unstable angina as the most significant correlate of PDGF β receptor tyrosine phosphorylation (F value 20.009, p < 0.0001). Conclusions - PDGF β receptor tyrosine phosphorylation in atherosclerotic lesions is increased compared with nonatherosclerotic arterial tissues. The association of PDGF β receptor tyrosine phosphorylation with immediate gain strongly correlates with vascular remodelling. PDGF β receptor tyrosine phosphorylation correlates with unstable angina pectoris.
AB - Background - Growth factors such as platelet derived growth factor (PDGF) have been postulated to be important mediators of neointimal proliferation observed in atherosclerotic plaques and restenotic lesions following coronary interventions. Binding of PDGF to its receptor results in intrinsic receptor tyrosine kinase activation and subsequent cellular migration, proliferation, and vascular contraction. Aims - To investigate whether the concentration of PDGF β receptor tyrosine phosphorylation obtained from directional coronary atherectomy (DCA) samples correlate with atherosclerotic plaque burden, the ability of diseased vessels to remodel, coronary risk factors, and clinical events. Methods - DCA samples from 59 patients and 15 non-atherosclerotic left internal thoracic arteries (LITA) were analysed for PDGF β receptor tyrosine phosphorylation content by receptor immunoprecipitation and antiphosphotyrosine western blot. The amount of PDGF β receptor phosphorylation was analysed in relation to angiographic follow up data and clinical variables. Results - PDGF β receptor tyrosine phosphorylation in the 59 DCA samples was greater than in the 15 non- atherosclerotic LITA (mean (SD) 0.84 (0.67) υ 0.17 (0.08) over a control standard, p < 0.0001). As evaluated by stepwise regression analysis, incorporation of both PDGF β receptor tyrosine phosphorylation and immediate gain correlated strongly (adjusted r2 = 0.579) with late loss, although PDGF β receptor tyramine phosphorylation alone correlated poorly with late loss. Multivariate regression analysis of coronary risk factors and clinical events revealed unstable angina as the most significant correlate of PDGF β receptor tyrosine phosphorylation (F value 20.009, p < 0.0001). Conclusions - PDGF β receptor tyrosine phosphorylation in atherosclerotic lesions is increased compared with nonatherosclerotic arterial tissues. The association of PDGF β receptor tyrosine phosphorylation with immediate gain strongly correlates with vascular remodelling. PDGF β receptor tyrosine phosphorylation correlates with unstable angina pectoris.
KW - Atherosclerosis
KW - Directional coronary atherectomy
KW - PDGF receptors
KW - Restenosis
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U2 - 10.1136/hrt.79.4.400
DO - 10.1136/hrt.79.4.400
M3 - Article
C2 - 9616351
AN - SCOPUS:0031958887
SN - 1355-6037
VL - 79
SP - 400
EP - 406
JO - Heart
JF - Heart
IS - 4
ER -