Universal marker and detection tool for human sarcoma circulating tumor cells

Arun Satelli, Abhisek Mitra, Jeffry J. Cutrera, Marcos Devarie, Xueqing Xia, Davis R. Ingram, Denada Dibra, Neeta Somaiah, Keila E. Torres, Vinod Ravi, Joseph A. Ludwig, Eugenie S. Kleinerman, Shulin Li

Research output: Contribution to journalArticlepeer-review

127 Scopus citations

Abstract

To date, no specific marker exists for the detection of circulating tumor cells (CTC) from different types of sarcomas, though tools are available for detection of CTCs in peripheral blood of patients with cancer for epithelial cancers. Here, we report cell-surface vimentin (CSV) as an exclusive marker on sarcoma CTC regardless of the tissue origin of the sarcoma as detected by a novel monoclonal antibody. Utilizing CSV as a probe, we isolated and enumerated sarcoma CTCs with high sensitivity and specificity from the blood of patients bearing different types of sarcoma, validating their phenotype by single cell genomic amplification, mutation detection, and FISH. Our results establish the first universal and specific CTC marker described for enumerating CTCs from different types of sarcoma, thereby providing a key prognosis tool to monitor cancer metastasis and relapse.

Original languageEnglish (US)
Pages (from-to)1645-1650
Number of pages6
JournalCancer Research
Volume74
Issue number6
DOIs
StatePublished - Mar 15 2014

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

MD Anderson CCSG core facilities

  • Monoclonal Antibody Facility
  • Advanced Technology Genomics Core
  • Flow Cytometry and Cellular Imaging Facility
  • Cytogenetics and Cell Authentication Core
  • Clinical Trials Office

Fingerprint

Dive into the research topics of 'Universal marker and detection tool for human sarcoma circulating tumor cells'. Together they form a unique fingerprint.

Cite this