Up-regulation of BRAF activated non-coding RNA is associated with radiation therapy for lung cancer

Jian xiang Chen, Ming Chen, Yuan da Zheng, Sheng ye Wang, Zhu ping Shen

    Research output: Contribution to journalArticle

    13 Scopus citations

    Abstract

    Radiation therapy has become more effective in treating primary tumors, such as lung cancer. Recent evidence suggested that BRAF activated non-coding RNAs (BANCR) play a critical role in cellular processes and are found to be dysregulated in a variety of cancers. The clinical significance of BANCR in radiation therapy, and its molecular mechanisms controlling tumor growth are unclear. In the present study, C57BL/6 mice were inoculated Lewis lung cancer cells and exposed to radiation therapy, then BANCR expression was analyzed using qPCR. Chromatin immunoprecipitation and western blot were performed to calculate the enrichment of histone acetylation and HDAC3 protein levels in Lewis lung cancer cells, respectively. MTT assay was used to evaluate the effects of BANCR on Lewis lung cancer cell viability. Finally, we found that BANCR expression was significantly increased in C57BL/6 mice receiving radiation therapy (P < 0.05) compared with control group. Additionally, knockdown of BANCR expression was associated with larger tumor size in C57BL/6 mice inoculated Lewis lung cancer cells. Histone deacetylation was observed to involve in the regulation of BANCR in Lewis lung cancer cells. Moreover, over expression HDAC3 reversed the effect of rays on BANCR expression. MTT assay showed that knockdown of BANCR expression promoted cell viability surviving from radiation. In conclusion, these findings indicated that radiation therapy was an effective treatment for lung cancer, and it may exert function through up-regulation BANCR expression.

    Original languageEnglish (US)
    Pages (from-to)79-83
    Number of pages5
    JournalBiomedicine and Pharmacotherapy
    Volume71
    DOIs
    StatePublished - Apr 1 2015

    Keywords

    • BRAF activated non-coding RNA
    • HDAC3
    • Histone acetylation
    • Lewis lung cancer cell
    • Lung cancer
    • Radiation therapy

    ASJC Scopus subject areas

    • Pharmacology

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