Upregulation of stem cell genes in multidrug resistant K562 leukemia cells

Gustav Lehne, Unn Hilde Grasmo-Wendler, Jeanne Marie Berner, Leonardo A. Meza-Zepeda, Birgitte Lid Adamsen, Aksel Flack, Andrew Reiner, Ole Petter Fraas Clausen, Eivind Hovig, Ola Myklebost

    Research output: Contribution to journalArticle

    22 Scopus citations

    Abstract

    The transmembrane transporter P-glycoprotein (P-gp) encoded by ABCB1, is one major cause for multidrug resistance (MDR). We compared the genomic profile and gene expression pattern of the P-gp positive K562VCR cells with parental P-gp negative K562wt cells. In K562VCR array CGH revealed amplification of ABCB1, ABCB4, ABCB5 and SEMA3D, whereas expression microarrays demonstrated upregulation of stem cell genes (e.g. KIT and HOXB4), anti-apoptotic genes (e.g. IGF1R and CCNG1), and downregulation of pro-apoptotic genes (e.g. CASP4, 6 and 7). Thus, K562VCR cells disclose stem cell characteristics including a range of drug resistance mechanisms possibly attained as a stem cell program switched on en bloc.

    Original languageEnglish (US)
    Pages (from-to)1379-1385
    Number of pages7
    JournalLeukemia Research
    Volume33
    Issue number10
    DOIs
    StatePublished - Oct 1 2009

    Keywords

    • ABCB1
    • ABCB4
    • ABCB5
    • Gene amplification
    • Multidrug resistance
    • SEMA3D
    • Stem cells

    ASJC Scopus subject areas

    • Hematology
    • Oncology
    • Cancer Research

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  • Cite this

    Lehne, G., Grasmo-Wendler, U. H., Berner, J. M., Meza-Zepeda, L. A., Adamsen, B. L., Flack, A., Reiner, A., Clausen, O. P. F., Hovig, E., & Myklebost, O. (2009). Upregulation of stem cell genes in multidrug resistant K562 leukemia cells. Leukemia Research, 33(10), 1379-1385. https://doi.org/10.1016/j.leukres.2009.03.028