Use of monoclonal antibodies in immuno-electron microscopy for the determination of subunit stoichiometry in oligomeric enzymes. There are three α-subunits in the F1-ATPase of Escherichia coli

H. Lünsdorf; K. Ehrig; P. Friedl; H. U. Schairer

doi:10.1016/0022-2836(84)90408-X

Use of monoclonal antibodies in immuno-electron microscopy for the determination of subunit stoichiometry in oligomeric enzymes. There are three α-subunits in the F₁-ATPase of Escherichia coli

H. Lünsdorf
, K. Ehrig
, P. Friedl
, H. U. Schairer

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

The subunit stoichiometry of oligomeric enzymes can be determined by immuno-electron microscopy using monoclonal antibodies against the individual subunits. Monoclonal antibodies against native F₁-ATPase of Escherichia coli were prepared that were specific for the α-subunit. The immune complexes of F₁ and monoclonal antibodies were isolated. Electron microscopy revealed the presence of three irnmunoglobulins per molecule of F₁-ATPase. This unequivocally demonstrates an α₃ stoichiometry for the F₁-ATPase of E. coli.

Original language	English (US)
Pages (from-to)	131-136
Number of pages	6
Journal	Journal of Molecular Biology
Volume	173
Issue number	1
DOIs	https://doi.org/10.1016/0022-2836(84)90408-X
State	Published - Feb 15 1984
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Structural Biology
Molecular Biology

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10.1016/0022-2836(84)90408-X

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Use of monoclonal antibodies in immuno-electron microscopy for the determination of subunit stoichiometry in oligomeric enzymes. There are three α-subunits in the F₁-ATPase of Escherichia coli. / Lünsdorf, H.; Ehrig, K.; Friedl, P. et al.
In: Journal of Molecular Biology, Vol. 173, No. 1, 15.02.1984, p. 131-136.

Research output: Contribution to journal › Article › peer-review

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AU - Friedl, P.

AU - Schairer, H. U.

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Use of monoclonal antibodies in immuno-electron microscopy for the determination of subunit stoichiometry in oligomeric enzymes. There are three α-subunits in the F₁-ATPase of Escherichia coli

Abstract

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