Using aptamers to study protein-protein interactions

Parag Parekh, Jennifer Martin, Yan Chen, Dalia Colon, Hui Wang, Weihong Tan

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

The emerging science of systems biology focuses on the systematic study of complex interactions in whole biological systems. A systemic, or integrative, methodology is employed as the chief means of discovering new properties and understanding the aggregate of processes that occur in a biological system. Accordingly, the Human Genome Project has provided a complete map of genes and resultant proteins corresponding to their function. Protein-protein interactions are important pieces of this biological tapestry, and understanding how they work cooperatively in a cell will result in a better understanding of the whole organism. To accomplish this objective, we report the use of DNA/RNA aptamers as a novel tool for the study and elucidation of protein-protein interactions, both in vivo and in vitro.

Original languageEnglish (US)
Title of host publicationProtein - Protein Interaction
EditorsMeike Werther, Harald Seitz
Pages177-194
Number of pages18
DOIs
StatePublished - 2008

Publication series

NameAdvances in Biochemical Engineering/Biotechnology
Volume110
ISSN (Print)0724-6145

Keywords

  • Aptamers
  • FRET
  • Fluorescence anisotropy
  • Protein-protein interactions

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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