Using drosophila larvae to study epidermal wound closure and inflammation

Sirisha Burra, Yan Wang, Amanda R. Brock, Michael J. Galko

Research output: Chapter in Book/Report/Conference proceedingChapter

17 Scopus citations

Abstract

This methods chapter describes two methods for creating epithelial wounds in Drosophila larvae: pinch and puncture wounding. It also covers protocols for visualizing epithelial wounds, either in a dissected whole mount preparation or, using transgenic reporter larvae, in a live whole mount preparation. Finally, useful transgenic lines for live genetic screening of genes required for wound closure or inflammation are described.

Original languageEnglish (US)
Title of host publicationWound Regeneration and Repair
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages449-461
Number of pages13
ISBN (Print)9781627035040
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1037
ISSN (Print)1064-3745

Keywords

  • Drosophila
  • Genetic screening
  • Inflammation
  • Transgenic reporters
  • Wound healing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Using drosophila larvae to study epidermal wound closure and inflammation'. Together they form a unique fingerprint.

Cite this