TY - JOUR
T1 - Using yeast two-hybrid system to identify ECRG2 associated proteins and their possible interactions with ECRG2 gene
AU - Cui, Yong Ping
AU - Wang, Jian Bo
AU - Zhang, Xin Yu
AU - Bi, Mei Xia
AU - Guo, Li Ping
AU - Lu, Shih Hsin
PY - 2003/9/15
Y1 - 2003/9/15
N2 - Aim: To identify esophageal cancer related gene2 (ECRG2) associated proteins and their possible interactions with ECRG2 gene. Methods: In the yeast forward two-hybrid system, ECRG2 was fused with the DNA-binding domain (DBD) of Gal4 and human fetal liver cDNA library was fused with the transcriptional activation domain (AD) of Gal4. We performed a high-stringency scale procedure to screen ECRG2 against human fetal liver cDNA library and characterized positives by sequence analysis. Results: We found the following 9 putatively associated proteins. They were metallothionein2A, metallothioneinlH, metallothionein1G, ferritin, erythrocyte membrane protein band4.2, mitochondrial ribosomal protein S12, hypothetical protein FLJ10101, and a novel gene whose cDNA was found to have no strong homology to any other previously characterized gene whose DDBJ/EMBL/GenBank accession number is AF22192 mapped to human chromosome 14q31. Conclusion: MT, a potential interaction partner for ECRG2, might be involved in the regulation of cell proliferation and apoptosis, and in various physiological processes. Determination of a reliability score for each single protein-protein interaction, especially interaction of ECRG2 and MT, permits the assignment of ECRG2 and unannotated proteins to biological pathways. A further understanding of the association between ECRG2 and MT should facilitate the functions of ECRG2 gene.
AB - Aim: To identify esophageal cancer related gene2 (ECRG2) associated proteins and their possible interactions with ECRG2 gene. Methods: In the yeast forward two-hybrid system, ECRG2 was fused with the DNA-binding domain (DBD) of Gal4 and human fetal liver cDNA library was fused with the transcriptional activation domain (AD) of Gal4. We performed a high-stringency scale procedure to screen ECRG2 against human fetal liver cDNA library and characterized positives by sequence analysis. Results: We found the following 9 putatively associated proteins. They were metallothionein2A, metallothioneinlH, metallothionein1G, ferritin, erythrocyte membrane protein band4.2, mitochondrial ribosomal protein S12, hypothetical protein FLJ10101, and a novel gene whose cDNA was found to have no strong homology to any other previously characterized gene whose DDBJ/EMBL/GenBank accession number is AF22192 mapped to human chromosome 14q31. Conclusion: MT, a potential interaction partner for ECRG2, might be involved in the regulation of cell proliferation and apoptosis, and in various physiological processes. Determination of a reliability score for each single protein-protein interaction, especially interaction of ECRG2 and MT, permits the assignment of ECRG2 and unannotated proteins to biological pathways. A further understanding of the association between ECRG2 and MT should facilitate the functions of ECRG2 gene.
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U2 - 10.3748/wjg.v9.i9.1892
DO - 10.3748/wjg.v9.i9.1892
M3 - Article
C2 - 12970870
AN - SCOPUS:0141829874
SN - 1007-9327
VL - 9
SP - 1892
EP - 1896
JO - World journal of gastroenterology
JF - World journal of gastroenterology
IS - 9
ER -