Utility of NKX3.1 Immunostaining in the Detection of Metastatic Prostatic Carcinoma on Fine-Needle Aspiration Smears

Qiong Gan; Cicily T. Joseph; Ming Guo; Miao Zhang; Xiaoping Sun; Yun Gong

doi:10.1093/ajcp/aqz063

Utility of NKX3.1 Immunostaining in the Detection of Metastatic Prostatic Carcinoma on Fine-Needle Aspiration Smears

Qiong Gan, Cicily T. Joseph, Ming Guo, Miao Zhang, Xiaoping Sun, Yun Gong

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Objectives: NK3 homeobox 1 (NKX3.1) has been increasingly used to diagnose metastatic prostatic carcinoma in histologic samples. However, its utility and reliability in cytologic direct smears have not been studied. Methods: A total of 59 fine-needle aspiration (FNA) cases with a definitive diagnosis of metastatic carcinoma from the prostate were included. The cases were grouped based on different Gleason score in their corresponding primary tumors and morphologic variants. For each case, tumor cells were immunostained with NKX3.1, prostate-specific antigen (PSA), and prostatic acid phosphatase (PAP) on cell-transferred smears. Results: NKX3.1 was strongly and diffusely positive in all 40 metastatic prostatic adenocarcinomas, including those with ductal features, but negative for the 19 small cell carcinoma (SmCC) cases. NKX3.1 had a better detection rate than PSA (13/50, 26%) and PAP (0/47, 0%). Conclusions: NKX3.1 immunostaining on FNA smears is highly reliable for detecting metastatic prostatic carcinomas of conventional and ductal types but not for SmCC.

Original language	English (US)
Pages (from-to)	495-501
Number of pages	7
Journal	American journal of clinical pathology
Volume	152
Issue number	4
DOIs	https://doi.org/10.1093/ajcp/aqz063
State	Published - Sep 9 2019

Keywords

Fine-needle aspiration
Metastatic prostatic carcinoma
NKX3.1
Smeared slide

ASJC Scopus subject areas

Pathology and Forensic Medicine

MD Anderson CCSG core facilities

Tissue Biospecimen and Pathology Resource

Access to Document

10.1093/ajcp/aqz063

Cite this

@article{d4bde6e265094b2dafdec3845a52b49a,

title = "Utility of NKX3.1 Immunostaining in the Detection of Metastatic Prostatic Carcinoma on Fine-Needle Aspiration Smears",

abstract = "Objectives: NK3 homeobox 1 (NKX3.1) has been increasingly used to diagnose metastatic prostatic carcinoma in histologic samples. However, its utility and reliability in cytologic direct smears have not been studied. Methods: A total of 59 fine-needle aspiration (FNA) cases with a definitive diagnosis of metastatic carcinoma from the prostate were included. The cases were grouped based on different Gleason score in their corresponding primary tumors and morphologic variants. For each case, tumor cells were immunostained with NKX3.1, prostate-specific antigen (PSA), and prostatic acid phosphatase (PAP) on cell-transferred smears. Results: NKX3.1 was strongly and diffusely positive in all 40 metastatic prostatic adenocarcinomas, including those with ductal features, but negative for the 19 small cell carcinoma (SmCC) cases. NKX3.1 had a better detection rate than PSA (13/50, 26%) and PAP (0/47, 0%). Conclusions: NKX3.1 immunostaining on FNA smears is highly reliable for detecting metastatic prostatic carcinomas of conventional and ductal types but not for SmCC.",

keywords = "Fine-needle aspiration, Metastatic prostatic carcinoma, NKX3.1, Smeared slide",

author = "Qiong Gan and Joseph, {Cicily T.} and Ming Guo and Miao Zhang and Xiaoping Sun and Yun Gong",

note = "Funding Information: Corresponding author: Yun Gong, MD; ygong@mdanderson.org. Acknowledgments: We thank Tamara K. Locke in the Department of Scientific Publications at The University of Texas MD Anderson Cancer Center for editing the manuscript. The study was supported by the faculty research fund of The University of Texas MD Anderson Cancer Center (to Y.G. and X.S.). Publisher Copyright: {\textcopyright} 2019 American Society for Clinical Pathology, 2019. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.",

year = "2019",

month = sep,

day = "9",

doi = "10.1093/ajcp/aqz063",

language = "English (US)",

volume = "152",

pages = "495--501",

journal = "American journal of clinical pathology",

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publisher = "American Society of Clinical Pathologists",

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T1 - Utility of NKX3.1 Immunostaining in the Detection of Metastatic Prostatic Carcinoma on Fine-Needle Aspiration Smears

AU - Gan, Qiong

AU - Joseph, Cicily T.

AU - Guo, Ming

AU - Zhang, Miao

AU - Sun, Xiaoping

AU - Gong, Yun

N1 - Funding Information: Corresponding author: Yun Gong, MD; ygong@mdanderson.org. Acknowledgments: We thank Tamara K. Locke in the Department of Scientific Publications at The University of Texas MD Anderson Cancer Center for editing the manuscript. The study was supported by the faculty research fund of The University of Texas MD Anderson Cancer Center (to Y.G. and X.S.). Publisher Copyright: © 2019 American Society for Clinical Pathology, 2019. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

PY - 2019/9/9

Y1 - 2019/9/9

N2 - Objectives: NK3 homeobox 1 (NKX3.1) has been increasingly used to diagnose metastatic prostatic carcinoma in histologic samples. However, its utility and reliability in cytologic direct smears have not been studied. Methods: A total of 59 fine-needle aspiration (FNA) cases with a definitive diagnosis of metastatic carcinoma from the prostate were included. The cases were grouped based on different Gleason score in their corresponding primary tumors and morphologic variants. For each case, tumor cells were immunostained with NKX3.1, prostate-specific antigen (PSA), and prostatic acid phosphatase (PAP) on cell-transferred smears. Results: NKX3.1 was strongly and diffusely positive in all 40 metastatic prostatic adenocarcinomas, including those with ductal features, but negative for the 19 small cell carcinoma (SmCC) cases. NKX3.1 had a better detection rate than PSA (13/50, 26%) and PAP (0/47, 0%). Conclusions: NKX3.1 immunostaining on FNA smears is highly reliable for detecting metastatic prostatic carcinomas of conventional and ductal types but not for SmCC.

AB - Objectives: NK3 homeobox 1 (NKX3.1) has been increasingly used to diagnose metastatic prostatic carcinoma in histologic samples. However, its utility and reliability in cytologic direct smears have not been studied. Methods: A total of 59 fine-needle aspiration (FNA) cases with a definitive diagnosis of metastatic carcinoma from the prostate were included. The cases were grouped based on different Gleason score in their corresponding primary tumors and morphologic variants. For each case, tumor cells were immunostained with NKX3.1, prostate-specific antigen (PSA), and prostatic acid phosphatase (PAP) on cell-transferred smears. Results: NKX3.1 was strongly and diffusely positive in all 40 metastatic prostatic adenocarcinomas, including those with ductal features, but negative for the 19 small cell carcinoma (SmCC) cases. NKX3.1 had a better detection rate than PSA (13/50, 26%) and PAP (0/47, 0%). Conclusions: NKX3.1 immunostaining on FNA smears is highly reliable for detecting metastatic prostatic carcinomas of conventional and ductal types but not for SmCC.

KW - Fine-needle aspiration

KW - Metastatic prostatic carcinoma

KW - NKX3.1

KW - Smeared slide

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ER -

Utility of NKX3.1 Immunostaining in the Detection of Metastatic Prostatic Carcinoma on Fine-Needle Aspiration Smears

Abstract

Keywords

ASJC Scopus subject areas

MD Anderson CCSG core facilities

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